Structural and mechanistic insights into the action of Plasmodium falciparum spermidine synthase.

Abstract

Spermidine synthase is currently considered as a promising drug target in the malaria parasite, Plasmodium falciparum, due to the vital role of spermidine in the activation of the eukaryotic translation initiation factor (eIF5A) and cell proliferation. However, very limited information was available regarding the structure and mechanism of action of the protein at the start of this study. Structural and mechanistic insights of the P. falciparum spermidine synthase (PfSpdSyn) were obtained utilizing molecular dynamics simulations of a homology model based on the crystal structures of the Arabidopsis thaliana and Thermotoga maritima homologues. Our data are supported by in vitro site-directed mutagenesis of essential residues as well as by a crystal structure of the protein that became available recently. We provide, for the first time, dynamic evidence for the mechanism of the aminopropyltransferase action of PfSpdSyn. This characterization of the structural and mechanistic properties of the PfSpdSyn as well as the elucidation of the active site residues involved in substrate, product, and inhibitor interactions paves the way toward inhibitor selection or design of parasite-specific inhibitors.

• Authors: Pieter B Burger, Lyn-Marie Birkholtz, Fourie Joubert, Nashya Haider, Rolf D Walter, Abraham I Louw
• Journal: Bioorganic & medicinal chemistry (Bioorg Med Chem) Vol. 15 Issue 4 Pg. 1628-37 (Feb 15 2007) ISSN: 0968-0896 [Print] England
• PMID: 17196392 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Peptide Initiation Factors
• Protozoan Proteins
• RNA-Binding Proteins
• eukaryotic translation initiation factor 5A
• Spermidine Synthase

Topics

• Animals
• Binding Sites
• Cell Proliferation
• Computer Simulation
• Motion
• Mutagenesis, Site-Directed
• Peptide Initiation Factors (physiology)
• Plasmodium falciparum (enzymology)
• Protozoan Proteins (chemistry, genetics)
• RNA-Binding Proteins (physiology)
• Spermidine Synthase (chemistry, genetics)