[Fluorescently labeled differentiating myelopeptide-4: specific binding to and penetration into target cells].

Abstract

Myelopeptide-4 (MP-4) (Phe-Arg-Pro-Arg-Ile-Met-Thr-Pro), inducing the terminal differentiation of HL-60 leukemia cells, was labeled with fluorescein isothiocyanate. The specific binding of this modified peptide to the surface of HL-60 cells and its ability to penetrate into the cells were studied. It was shown by cytometry and confocal microscopy to be bound on the HL-60 cell surface, to penetrate into their cytoplasm, and finally to concentrate around the cell nucleus. These phenomena are probably necessary for the exhibition of MP-4 differentiating activity.

• Authors: S A Gur'ianov, E A Kirilina, S V Khaĭdukov, N I Suvorov, I M Molotkovskaia, A A Mikhaĭlova
• Journal: Bioorganicheskaia khimiia (Bioorg Khim) 2006 Nov-Dec Vol. 32 Issue 6 Pg. 574-8 ISSN: 0132-3423 [Print] Russia (Federation)
• PMID: 17180907 (Publication Type: English Abstract, Journal Article)

Chemical References

• Fluorescent Dyes
• Oligopeptides
• phenylalanyl-arginyl-prolyl-arginyl-isoleucyl-methionyl-threonyl-proline
• Fluorescein-5-isothiocyanate

Topics

• Cell Differentiation (drug effects)
• Cytoplasm (metabolism)
• Fluorescein-5-isothiocyanate (chemistry, metabolism, pharmacology)
• Fluorescent Dyes (chemistry, metabolism, pharmacology)
• HL-60 Cells
• Humans
• Microscopy, Confocal
• Microscopy, Fluorescence
• Oligopeptides (chemistry, metabolism, pharmacology)
• Protein Transport (drug effects)