Restricted replication and lysosomal trafficking of yellow fever 17D vaccine virus in human dendritic cells.

Abstract

The yellow fever virus attenuated 17D vaccine strain is a safe and effective vaccine and a valuable model system for evaluating immune responses against attenuated viral variants. This study compared the in vitro interactions of the commercially available yellow fever vaccine (YF-VAX), Dengue virus and the live-attenuated dengue vaccine PDK50 with dendritic cells (DCs), the main antigen-presenting cells at the initiation of immune responses. Similar to PDK50, infection with YF-VAX generated activated DCs; however, for YF-VAX, activation occurred with limited intracellular virus replication. The majority of internalized virus co-localized with endolysosomal markers within 90 min, suggesting that YF-VAX is processed rapidly in DCs. These results indicate that restricted virus replication and lysosomal compartmentalization may be important contributing factors to the success of the YF-VAX vaccine.

• Authors: Dupeh R Palmer, Stefan Fernandez, John Bisbing, Kristina K Peachman, Mangala Rao, Dave Barvir, Vicky Gunther, Timothy Burgess, Yukari Kohno, R Padmanabhan, Wellington Sun
• Journal: The Journal of general virology (J Gen Virol) Vol. 88 Issue Pt 1 Pg. 148-156 (Jan 2007) ISSN: 0022-1317 [Print] England
• PMID: 17170447 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Yellow Fever Vaccine

Topics

• Animals
• Chlorocebus aethiops
• Dendritic Cells (virology)
• Humans
• Lysosomes (metabolism, virology)
• Vero Cells
• Virus Replication
• Yellow Fever Vaccine (adverse effects, immunology)
• Yellow fever virus (immunology, physiology)