Use of lactophenol cotton blue mounts of corneal scrapings as an aid to the diagnosis of mycotic keratitis.
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| Abstract |
Lactophenol cotton blue (LPCB) mounts of corneal ulcer scrapings were assessed as a diagnostic tool in cases of mycotic keratitis over a period of 15 months. We investigated 568 cases of ulcerative keratitis by microbiological techniques consisting of direct microscopic examination of LPCB mounts and Gram-stained smears as well as culture of material scraped from the ulcer. Fungi were isolated in large numbers on multiple media from the corneal scrapings of 179 patients (culture-proven mycotic keratitis). Direct microscopic examination of LPCB mounts of corneal scrapings yielded positive results in 140 (78%) of 179 culture-positive patients and negative results in 371 (95%) of 389 culture-negative patients. There was a significant difference between the percentage of positive results obtained by LPCB mounts and by Gram-stained smears in the culture-positive cases. The LPCB mount was positive in greater than 80% of cases of keratitis due to Fusarium spp. and Aspergillus spp. The LPCB mount is strongly recommended as a simple, rapid, inexpensive, and sensitive diagnostic technique in cases of mycotic keratitis.
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| Authors | P A Thomas, T Kuriakose, M P Kirupashanker, V S Maharajan |
| Journal | Diagnostic microbiology and infectious disease (Diagn Microbiol Infect Dis) 1991 May-Jun Vol. 14 Issue 3 Pg. 219-24 ISSN: 0732-8893 [Print] United States |
| PMID | 1716191 (Publication Type: Comparative Study, Journal Article) |
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