Angelan isolated from Angelica gigas Nakai induces dendritic cell maturation through toll-like receptor 4.

Abstract

During the evolution of neoplastic diseases, dendritic cell (DC) functions are usually attenuated, and this presents a problem to DC-based immunotherapies against cancer. Here, we investigated the effects of angelan, an acidic polysaccharide isolated from Angelica gigas Nakai, on DC maturation. Angelan efficiently increased the maturation of tlr4(+/+) DCs from C57BL/6 and C3H/HeN mice, but not tlr4(-/-) DCs from C3H/HeJ mice. Phenotypic maturation was confirmed by the elevated expressions of CD80, CD86, and MHC-class II molecules, and functional maturation by increased IL-12 production, enhanced allogenic T cell stimulation, and decreased endocytosis. Angelan was found to activate ERK and NF-kappaB, which are signaling molecules down-stream of toll-like receptor-4 (TLR4) receptors. Angelan-treated mature DC more effectively inhibited B16F10 tumor growth than immature DCs in syngenic murine tumor model. These results indicate that angelan induces DC maturation via TLR4 signaling pathways and suggest the possible use of angelan in DC-based immunotherapies.

• Authors: Jee Youn Kim, Yeo Dae Yoon, Ji Mi Ahn, Jong Soon Kang, Song-Kyu Park, Kiho Lee, Kyung Bin Song, Hwan Mook Kim, Sang-Bae Han
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 7 Issue 1 Pg. 78-87 (Jan 2007) ISSN: 1567-5769 [Print] Netherlands
• PMID: 17161820 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Polysaccharides
• Tlr4 protein, mouse
• Toll-Like Receptor 4
• angelan

Topics

• Angelica (chemistry)
• Animals
• Cell Differentiation (drug effects)
• Cells, Cultured
• Cytokines (immunology)
• Dendritic Cells (cytology, drug effects, immunology)
• Endocytosis (drug effects)
• Female
• Lymphocyte Culture Test, Mixed
• Melanoma, Experimental (drug therapy)
• Mice
• Mice, Inbred BALB C
• Mice, Inbred C3H
• Mice, Inbred C57BL
• Polysaccharides (isolation & purification, pharmacology)
• Spleen (cytology)
• T-Lymphocytes (immunology)
• Toll-Like Receptor 4 (immunology)
• Xenograft Model Antitumor Assays