Magnetic resonance imaging at 1.5 T with immunospecific contrast agent in vitro and in vivo in a xenotransplant model.

Abstract

OBJECT:

Demonstrating the feasibility of magnetic resonance imaging (MRI) at 1.5 T of ultrasmall particle iron oxide (USPIO)-antibody bound to tumor cells in vitro and in a murine xenotransplant model.

METHODS:

Human D430B cells or Raji Burkitt lymphoma cells were incubated in vitro with different amounts of commercially available USPIO-anti-CD20 antibodies and cell pellets were stratified in a test tube. For in vivo studies, D430B cells and Raji lymphoma cells were inoculated subcutaneously in immunodeficient mice. MRI at 1.5 T was performed with T1-weighted three-dimensional fast field echo sequences (17/4.6/13 degrees ) and T2-weighted three-dimensional fast-field echo sequences (50/12/7 degrees ). For in vivo studies MRI was performed before and 24 h after USPIO-anti-CD20 administration.

RESULTS:

USPIO-anti-CD20-treated D430B cells, showed a dose-dependent decrease in signal intensity (SI) on T2*-weighted images and SI enhancement on T1-weighted images in vitro. Raji cells showed lower SI changes, in accordance to the fivefold lower expression of CD20 on Raji with respect to D430B cells. In vivo 24 h after USPIO-anti-CD20 administration, both tumors showed an inhomogeneous decrease of SI on T2*-weighted images and SI enhancement on T1-weighted images.

CONCLUSIONS:

MRI at 1.5 T is able to detect USPIO-antibody conjugates targeting a tumor-associated antigen in vitro and in vivo.

• Authors: G Baio, M Fabbi, D de Totero, S Ferrini, M Cilli, L E Derchi, C E Neumaier
• Journal: Magma (New York, N.Y.) (MAGMA) Vol. 19 Issue 6 Pg. 313-20 (Dec 2006) ISSN: 0968-5243 [Print] Germany
• PMID: 17160691 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Antibodies, Monoclonal, Murine-Derived
• Antigens, CD20
• Contrast Media
• Dextrans
• Magnetite Nanoparticles
• Oxides
• ferumoxtran-10
• Rituximab
• Iron
• Ferrosoferric Oxide

Topics

• Animals
• Antibodies, Monoclonal (immunology)
• Antibodies, Monoclonal, Murine-Derived
• Antigens, CD20 (immunology)
• Cell Line, Tumor
• Contrast Media
• Dextrans
• Disease Models, Animal
• Drug Delivery Systems (methods)
• Ferrosoferric Oxide
• Image Enhancement (methods)
• Iron
• Lymphoma (immunology, pathology)
• Magnetic Resonance Imaging (methods)
• Magnetite Nanoparticles
• Mice
• Oxides
• Rituximab
• Transplantation, Heterologous