| Abstract | OBJECTIVE: To clarify the dose-related effects of direct hemoperfusion using a cytokine adsorbent column (CTR) on the mortality and inflammatory responses to endotoxin-induced shock in rats. DESIGN: Prospective and randomized study. SETTING: University research laboratory. SUBJECTS: Male Wistar rats. INTERVENTIONS: Forty-eight rats were injected intravenously with Escherichia coli endotoxin (15 mg/kg over 2 min), and then were randomly allocated to one of the following four groups (n = 12 per group): control group, treated without CTR for 120 min; quarter-dose treatment group, treated with CTR 0.25 ml for 120 min; half-dose treatment group, treated with CTR 0.50 ml; and full-dose treatment group, treated with CTR 1.0 ml. MEASUREMENTS AND RESULTS: Hemodynamics and arterial blood gases were recorded, and mortality and plasma cytokine concentrations were calculated for the 8-h observation period. The mortality rates 8 h after endotoxin injection were 92%, 58%, 42% and 17% for the control column, quarter-dose, half-dose, and full-dose CTR groups, respectively. The increases in plasma cytokine concentrations were smaller in the half-dose and full-dose CTR treatment groups than in the endotoxemic group. CONCLUSIONS: The present study showed that CTR treatment dose-dependently decreased the mortality rate and inhibited inflammatory responses in vivo. |
| Authors | Takumi Taniguchi, Akihide Kurita, Chisui Mukawa, Ken Yamamoto, Hideo Inaba
(Affiliation: Department of Emergency and Critical Care Medicine, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, 920-8641, Kanazawa, Japan. takutaniyan at yahoo.co.jp)
|
| Journal | Intensive care medicine
(Intensive Care Med)
Vol. 33
Issue 3
Pg. 529-33
(Mar 2007)
ISSN: 0342-4642 United States |
| PMID | 17160419
(Publication Type: Evaluation Studies, Journal Article)
|
| Chemical References |
- Cytokines
- Interleukin-6
- Tumor Necrosis Factor-alpha
|
| Topics |
- Analysis of Variance
- Animals
- Cytokines
(blood)
- Endotoxemia
(blood, therapy)
- Hemoperfusion
(methods)
- Interleukin-6
- Male
- Prospective Studies
- Random Allocation
- Rats
- Rats, Wistar
- Shock, Septic
(blood, therapy)
- Survival Analysis
- Tumor Necrosis Factor-alpha
|
