Abstract | CONTEXT: The Ewing family of tumors are often difficult to distinguish from other malignant small round cell tumors, but more than 90% have EWS-FLI1 chimeric transcript, which acts as a potential molecular diagnostic marker. OBJECTIVE: DESIGN: The initial diagnosis was made on core biopsy (n = 22) and open biopsy (n = 4) specimens by using morphology and immunohistochemistry and on fine-needle aspiration cytology ([FNAC], n = 6) specimens. EWS-FLI1 was detected by reverse transcriptase polymerase chain reaction on all 32 fresh FNAC samples and by fluorescence in situ hybridization on 16 paraffin blocks. RESULTS: CONCLUSIONS: The excellent correlation of routine histologic findings in Ewing family of tumors with results on immunohistochemistry and fluorescence in situ hybridization on archival material and reverse transcriptase polymerase chain reaction on fresh FNAC specimens underscores that the traditional observation on routine histologic examination is a time-tested tool. The diagnosis of Ewing family of tumors can be validated on archival material or fresh biopsy samples, including those obtained by FNAC.
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Authors | Nirmala A Jambhekar, Izhar N Bagwan, Prachi Ghule, Tanuja M Shet, Roshni F Chinoy, Sandhya Agarwal, Rupali Joshi, Pratibha S Amare Kadam |
Journal | Archives of pathology & laboratory medicine
(Arch Pathol Lab Med)
Vol. 130
Issue 12
Pg. 1813-8
(Dec 2006)
ISSN: 1543-2165 [Electronic] United States |
PMID | 17149955
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- DNA, Neoplasm
- EWS-FLI fusion protein
- Oncogene Proteins, Fusion
- Proto-Oncogene Protein c-fli-1
- RNA-Binding Protein EWS
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Topics |
- Adolescent
- Adult
- Biopsy, Fine-Needle
- Bone Neoplasms
(diagnosis)
- Carcinoma, Small Cell
(diagnosis)
- Child
- Child, Preschool
- DNA, Neoplasm
(analysis)
- Diagnosis, Differential
- Female
- Humans
- Immunohistochemistry
(methods)
- In Situ Hybridization, Fluorescence
- Infant
- Male
- Middle Aged
- Oncogene Proteins, Fusion
(analysis)
- Proto-Oncogene Protein c-fli-1
(analysis)
- RNA-Binding Protein EWS
- Reverse Transcriptase Polymerase Chain Reaction
(methods)
- Sarcoma, Ewing
(diagnosis)
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