Effects of sodium hyaluronate and methylprednisolone acetate on proteoglycan metabolism in equine articular chondrocytes treated with interleukin-1.

Abstract

OBJECTIVE:

To determine the effects of sodium hyaluronate (HA) in combination with methylprednisolone acetate (MPA) on interleukin-1 (IL-1)-induced inflammation in equine articular cartilage pellets.

SAMPLE POPULATION:

Chondrocytes collected from 7 horses euthanatized for problems unrelated to the musculoskeletal system.

PROCEDURES:

Chondrocyte pellets were treated with medium (negative control); medium containing IL-1 (positive control); or medium containing IL-1 with MPA only (0.05 or 0.5 mg/mL), HA only (0.2 or 2 mg/mL), or MPA (0.05 or 0.5 mg/mL) and HA (0.2 or 2 mg/mL) in combination. Proteoglycan (PG) synthesis was determined by incorporation of sulfur 35-labeled sodium sulfate into PGs. Glycosaminoglycan (GAG) content of the media and the pellets and total pellet DNA content were determined.

RESULTS:

Methylprednisolone acetate at 0.5 mg/mL caused an increase in PG synthesis, whereas HA had no effect alone. The combination of MPA, both 0.05 mg/mL and 0.5 mg/mL, with HA at 2 mg/mL increased PG synthesis, compared with IL-1-treated control. All treatment groups containing the high concentration of MPA (0.5 mg/mL) and the high concentration of HA (2.0 mg/mL) had pellets with increased GAG content. The addition of HA caused an increase in total GAG content in the media, regardless of MPA treatment. Cyclooxygenase-2 mRNA and aggrecan mRNA expression was significantly reduced with MPA treatment. Total pellet DNA content was unchanged by any treatment.

CONCLUSIONS AND CLINICAL RELEVANCE:

Our results indicate that MPA in combination with HA has beneficial effects on PG metabolism of IL-1-treated equine chondrocytes.

• Authors: Angela C Yates, Allison A Stewart, Christopher R Byron, Holly C Pondenis, Karissa M Kaufmann, Peter D Constable
• Journal: American journal of veterinary research (Am J Vet Res) Vol. 67 Issue 12 Pg. 1980-6 (Dec 2006) ISSN: 0002-9645 [Print] United States
• PMID: 17144797 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Adjuvants, Immunologic
• Anti-Inflammatory Agents
• Interleukin-1
• Proteoglycans
• Sulfur Radioisotopes
• Methylprednisolone Acetate
• Hyaluronic Acid
• Methylprednisolone

Topics

• Adjuvants, Immunologic (administration & dosage, pharmacology)
• Animals
• Anti-Inflammatory Agents (administration & dosage, pharmacology)
• Cells, Cultured
• Chondrocytes (cytology, drug effects, metabolism)
• Horses (metabolism)
• Hyaluronic Acid (administration & dosage, pharmacology)
• Inflammation (drug therapy)
• Interleukin-1 (toxicity)
• Methylprednisolone (administration & dosage, analogs & derivatives, pharmacology)
• Methylprednisolone Acetate
• Proteoglycans (analysis, drug effects, metabolism)
• Sulfur Radioisotopes (analysis)