| Abstract | OBJECTIVE: To investigate hepatocyte growth factor (HGF) primed fibroblasts and decorin application on skin and vocal fold fibroblasts in vitro and in vivo in rabbit vocal fold scar model. STUDY DESIGN AND SETTING: Vocal fold and skin fibroblasts underwent five in vitro treatment conditions: control, epidermal growth factor, HGF, both decorin and HGF, and decorin alone. Hyaluronic acid and collagen enzyme-linked immunosorbent assays were performed. In vivo, 12 rabbits underwent unilateral vocal fold stripping. Injured vocal folds were then injected with skin fibroblasts, HGF, HGF-primed fibroblasts and decorin, or decorin. Outcomes included histologic and lamina propria height analyses. RESULTS: In vitro, HGF increased hyaluronic acid synthesis in vocal fold fibroblasts (P<0.001). HGF and decorin treatment diminished collagen secretion (P<0.01). In vivo, histologic findings indicated minimal difference in collagen amount between treatment groups. CONCLUSION: HGF and decorin together may decrease collagen production by skin and vocal fold fibroblasts. Fibroblast transplantation into scarred vocal folds has equivocal benefit. |
| Authors | Priya Krishna, Clark A Rosen, Ryan C Branski, Alan Wells, Patricia A Hebda
(Affiliation: University of Pittsburgh Voice Center, Department of Otolaryngology, PA 15213, USA. krishnpd at upmc.edu)
|
| Journal | Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery
(Otolaryngol Head Neck Surg)
Vol. 135
Issue 6
Pg. 937-45
(Dec 2006)
ISSN: 0194-5998 United States |
| PMID | 17141088
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Collagen Type I
- Extracellular Matrix Proteins
- Proteoglycans
- decorin
- Epidermal Growth Factor
- Hepatocyte Growth Factor
- Hyaluronic Acid
|
| Topics |
- Animals
- Cells, Cultured
- Cicatrix
(drug therapy)
- Collagen Type I
(drug effects, metabolism)
- Disease Models, Animal
- Epidermal Growth Factor
(pharmacology, therapeutic use)
- Extracellular Matrix Proteins
(pharmacology, therapeutic use)
- Female
- Fibroblasts
- Hepatocyte Growth Factor
(pharmacology, therapeutic use)
- Hyaluronic Acid
(metabolism)
- Proteoglycans
(pharmacology, therapeutic use)
- Rabbits
- Skin
(pathology)
- Vocal Cords
(pathology)
|
