Sequence analysis of the nucleoprotein gene of Asian lineage peste des petits ruminants vaccine virus.

Abstract

The complete nucleotide sequence of the nucleocapsid (N) protein of the peste-des-petits ruminants vaccine virus (PPRV Sungri/96) belonging to the Asian lineage was determined. The gene was 1692 nucleotides in length and encoded a polypeptide of 525 amino acids. The PPRV Sungri/96 N gene has a nucleotide homology of 92% for PPRV Nigeria 75/1 to 55.5% for canine distemper virus. At amino acid level the homology was 94.1% with PPRV Nigeria 75/1, while with other morbilliviruses, PPRV Sungri/96 had only 71.4-64.9% amino acid identity. The phosphorylation prediction reveals eight conserved sites across morbilliviruses, whereas in the C-terminal portion of the protein the sites are not conserved. Phylogenetic analysis of different N proteins of morbilliviruses revealed five well-defined clusters as observed previously. To the best of our knowledge this is the first report describing the nucleocapsid gene sequence of PPRV Indian isolate.

• Authors: D Muthuchelvan, A Sanyal, V Balamurugan, P Dhar, S K Bandyopadhyay
• Journal: Veterinary research communications (Vet Res Commun) Vol. 30 Issue 8 Pg. 957-63 (Nov 2006) ISSN: 0165-7380 [Print] Netherlands
• PMID: 17139548 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Nucleoproteins
• Viral Vaccines

Topics

• Amino Acid Sequence
• Animals
• Asia
• Base Sequence
• Chlorocebus aethiops
• Molecular Sequence Data
• Nucleoproteins (genetics)
• Peste-des-Petits-Ruminants (virology)
• Peste-des-petits-ruminants virus (genetics)
• Phylogeny
• Vero Cells
• Viral Vaccines (genetics)