Identification of squamous cell carcinoma associated proteins by proteomics and loss of beta tropomyosin expression in esophageal cancer.

Abstract

AIM:

To assess the proteome of normal versus tumor tissue in squamous cell carcinoma of the esophagus (SCCE) in Iranian patients and compare our results with former reports by using proteomics.

METHODS:

Protein was extracted from normal and tumor tissues. Two dimensional electrophoresis was carried out and spots with differential expression were identified with mass spectrometry. RNA extraction and RT-PCR along with immunodetection were performed.

RESULTS:

Fourteen proteins were found whose expression levels differed in tumor compared to normal tissues. Mass spectrometric analysis resulted in the identification of beta-tropomyosin (TMbeta), myosin light chain 2 (and its isoform), myosin regulatory light chain 2, peroxyredoxin 2, annexin I and an unknown polypeptide as the down regulated polypeptides in tumor tissue. Heat shock protein 70 (HSP70), TPM4-ALK fusion oncoprotein 2, myosin light polypeptide 6, keratin I, GH16431p and calreticulin were the up-regulated polypeptides found in tumor tissue. Several of these proteins, such as TMbeta, HSP70, annexin I, calreticulin, TPM4-ALK and isoforms of myosins, have been well recognized in tumorigenesis of esophageal or other types of cancers.

CONCLUSION:

Our study not only supports the involvement of some of the formerly reported proteins in SCCE but also introduces additional proteins found to be lost in SCCE, including TMbeta.

• Authors: Ferdous-Rastgar Jazii, Zahra Najafi, Reza Malekzadeh, Thomas-P Conrads, Abed-Ali Ziaee, Christian Abnet, Mansour Yazdznbod, Ali-Asghar Karkhane, Ghasem-H Salekdeh
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 12 Issue 44 Pg. 7104-12 (Nov 28 2006) ISSN: 1007-9327 [Print] United States
• PMID: 17131471 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Proteins
• Tropomyosin

Topics

• Adult
• Aged
• Aged, 80 and over
• Amino Acid Sequence
• Carcinoma, Squamous Cell (genetics, metabolism)
• Electrophoresis, Gel, Two-Dimensional
• Esophageal Neoplasms (genetics, metabolism)
• Gene Expression Profiling
• Humans
• Middle Aged
• Molecular Sequence Data
• Proteins (isolation & purification)
• Proteomics
• Reverse Transcriptase Polymerase Chain Reaction
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• Tropomyosin (metabolism)