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The effects of hypoxia-ischemia on neutral amino acid transporters in the developing rat brain.

Abstract
The neutral amino acid transporters SNAT1-3 and ASCT1 play critical roles in the recycling of glutamine, and subsequently glutamate, via the glutamine-glutamate cycle. Hypoxia-ischemia was induced in rat pups using the Rice-Vannucci model. Brains were harvested at 1 h, 24 h and 7 days after ischemia. The expression of NAATs was evaluated using immunoblotting, real-time PCR, and immunohistochemistry. Results were compared with age-matched controls and shams. SNAT1 mRNA decreased at 1 h after injury in both hemispheres when compared with the control animals and correlated with a decrease in protein expression at 24 h in the hippocampus and cortex. SNAT1 protein expression increased globally at 7 days after injury and specifically in the hippocampus. Finally, SNAT2 and 3 demonstrated subtle changes in various brain regions after injury. These data suggest that neutral amino acid transporters remain largely intact after hypoxia-ischemia.
AuthorsAvital Leibovici, Candace Rossignol, Jennifer A Montrowl, Jeffery D Erickson, Helene Varoqui, Masahiko Watanabe, Farrukh A Chaudhry, May Kristin L Bredahl, Kevin J Anderson, Michael D Weiss
JournalDevelopmental neuroscience (Dev Neurosci) Vol. 29 Issue 3 Pg. 268-74 ( 2007) ISSN: 0378-5866 [Print] Switzerland
PMID17124376 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
CopyrightCopyright (c) 2006 S. Karger AG, Basel.
Chemical References
  • Amino Acid Transport System A
  • Amino Acid Transport System ASC
  • Amino Acid Transport Systems
  • Amino Acid Transport Systems, Neutral
  • Amino Acids, Neutral
  • Slc1a4 protein, rat
  • Slc38a1 protein, rat
  • Slc38a2 protein, rat
  • system N protein 1
Topics
  • Amino Acid Transport System A (genetics, metabolism)
  • Amino Acid Transport System ASC (genetics, metabolism)
  • Amino Acid Transport Systems (genetics, metabolism)
  • Amino Acid Transport Systems, Neutral (genetics, metabolism)
  • Amino Acids, Neutral (metabolism)
  • Animals
  • Brain (growth & development, physiology)
  • Gene Expression Regulation, Developmental
  • Hypoxia-Ischemia, Brain (metabolism, physiopathology)
  • Immunoblotting
  • Immunohistochemistry
  • Rats
  • Rats, Sprague-Dawley
  • Reverse Transcriptase Polymerase Chain Reaction

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