Abstract |
The neutral amino acid transporters SNAT1-3 and ASCT1 play critical roles in the recycling of glutamine, and subsequently glutamate, via the glutamine- glutamate cycle. Hypoxia- ischemia was induced in rat pups using the Rice-Vannucci model. Brains were harvested at 1 h, 24 h and 7 days after ischemia. The expression of NAATs was evaluated using immunoblotting, real-time PCR, and immunohistochemistry. Results were compared with age-matched controls and shams. SNAT1 mRNA decreased at 1 h after injury in both hemispheres when compared with the control animals and correlated with a decrease in protein expression at 24 h in the hippocampus and cortex. SNAT1 protein expression increased globally at 7 days after injury and specifically in the hippocampus. Finally, SNAT2 and 3 demonstrated subtle changes in various brain regions after injury. These data suggest that neutral amino acid transporters remain largely intact after hypoxia- ischemia.
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Authors | Avital Leibovici, Candace Rossignol, Jennifer A Montrowl, Jeffery D Erickson, Helene Varoqui, Masahiko Watanabe, Farrukh A Chaudhry, May Kristin L Bredahl, Kevin J Anderson, Michael D Weiss |
Journal | Developmental neuroscience
(Dev Neurosci)
Vol. 29
Issue 3
Pg. 268-74
( 2007)
ISSN: 0378-5866 [Print] Switzerland |
PMID | 17124376
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright (c) 2006 S. Karger AG, Basel. |
Chemical References |
- Amino Acid Transport System A
- Amino Acid Transport System ASC
- Amino Acid Transport Systems
- Amino Acid Transport Systems, Neutral
- Amino Acids, Neutral
- Slc1a4 protein, rat
- Slc38a1 protein, rat
- Slc38a2 protein, rat
- system N protein 1
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Topics |
- Amino Acid Transport System A
(genetics, metabolism)
- Amino Acid Transport System ASC
(genetics, metabolism)
- Amino Acid Transport Systems
(genetics, metabolism)
- Amino Acid Transport Systems, Neutral
(genetics, metabolism)
- Amino Acids, Neutral
(metabolism)
- Animals
- Brain
(growth & development, physiology)
- Gene Expression Regulation, Developmental
- Hypoxia-Ischemia, Brain
(metabolism, physiopathology)
- Immunoblotting
- Immunohistochemistry
- Rats
- Rats, Sprague-Dawley
- Reverse Transcriptase Polymerase Chain Reaction
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