New insight into the inhibition of the inflammatory response to experimental delayed-type hypersensitivity reactions in mice by scropolioside A.

Abstract

Scropolioside A, an iridoid isolated from Scrophularia auriculata ssp. pseudoauriculata, showed anti-inflammatory properties against different experimental models of delayed-type hypersensitivity. This iridoid reduced the oedema induced by oxazolone by 79% (72 h) at 0.5 mg/ear while reducing that induced by sheep red blood cells by 47% (18 h), 45% (24 h) and 36% (48 h) at 10 mg/kg. In vivo it reduced both oedema formation and cell infiltration whereas in vitro it reduced the proliferation of activated T-lymphocytes (IC50 of 67.74 microM). Treatment with scropolioside A (100 microM) 18 and 24 h after phytohemagglutinin stimulation increased the number of cells arrested in the subG(0) phase whereas treatment 3 h after stimulation clearly increased the number of cells that passed to the S phase. Scropolioside A also inhibited the production of prostaglandin E2, leukotriene B4, nitric oxide, interleukin-1beta, interleukin-2, interleukin-4, tumour necrosis factor-alpha and interferon-gamma, but had no effect on the production of interleukin-10. Moreover, it modified the expression of both nitric oxide synthase-2 and cyclooxygenase-2, as well as the activation of nuclear factor-kappaB in RAW 264.7 macrophages.

• Authors: Esperanza Bas, M Carmen Recio, Salvador Máñez, Rosa M Giner, José M Escandell, Concha López-Ginés, José-Luis Ríos
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 555 Issue 2-3 Pg. 199-210 (Jan 26 2007) ISSN: 0014-2999 [Print] Netherlands
• PMID: 17113578 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Allergens
• Anti-Inflammatory Agents
• Cytokines
• Glucosides
• Lipopolysaccharides
• Pyrans
• scropolioside A
• Oxazolone
• Leukotriene B4
• Pancreatic Elastase
• Caspase 3
• Dinoprostone

Topics

• Allergens
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Apoptosis (drug effects)
• Caspase 3 (metabolism)
• Cell Line
• Cytokines (biosynthesis)
• Dinoprostone (immunology)
• Ear (pathology)
• Edema (chemically induced)
• Erythrocytes (immunology)
• Female
• Glucosides (pharmacology)
• Humans
• Hypersensitivity, Delayed (chemically induced, drug therapy, immunology, pathology)
• Leukotriene B4 (immunology)
• Lipopolysaccharides
• Macrophages (drug effects, immunology)
• Mice
• Neutrophils (drug effects, immunology)
• Oxazolone
• Pancreatic Elastase (immunology)
• Pyrans (pharmacology)
• Sheep
• T-Lymphocytes (cytology, drug effects, immunology)