Evaluation of wound healing effect on skin-defect nude mice by using human dermis-derived mesenchymal stem cells.

Abstract

We report that human dermis-derived mesenchymal stem cells (hDMSCs) possess differentiation potential of epidermis facilitating wound healing in skin-defect nude mice in combination with the treatment using gelatin/thermosensitive poly N-isopropylacrylamide (pNIPAAm)/polypropylene (PP). The results showed that the rate of cell growth and wound recovery in the hDMSC and gelatin/pNIPAAm/PP-treated group was significantly greater than those in the gelatin/pNIPAAm/PP-treated only group (P < .01). The reepithelialization marker of human pan-cytokeratin was also significantly increased on days 14 and day 21 in the wound site of hDMSCs and gelatin/pNIPAAm/PP-treated group. Furthermore, the stem cell marker of human CD13 gradually decreased during the period of wound healing. In sum, this novel method provided a transferring system for stem cell therapy, maintaining its temperature-sensitive property of easy peeling by lower temperature treatment.

• Authors: C-K Perng, H-H Ku, S-H Chiou, I-L Chen, F-T Tsai, Y-P Yang, K Y Chang, C-L Kao
• Journal: Transplantation proceedings (Transplant Proc) Vol. 38 Issue 9 Pg. 3086-7 (Nov 2006) ISSN: 0041-1345 [Print] United States
• PMID: 17112905 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Animals
• Dermis (cytology)
• Disease Models, Animal
• Humans
• Mesenchymal Stem Cell Transplantation
• Mice
• Mice, Nude
• Skin (pathology)
• Transplantation, Heterologous
• Wound Healing (physiology)