Cell cycle regulatory proteins in the liver in murine Trypanosoma cruzi infection.

Abstract	The liver is an important target of Trypanosoma cruzi infection. Infection of CD-1 mice with T. cruzi (Brazil strain) resulted in parasitism of the liver, primarily in sinusoidal and Kupffer cells. Immunoblot analysis revealed activation of extra cellular signal-regulated kinase (ERK) during the acute and subacute period of infection, but p38 mitogen activated kinase (MAPK) and JNK were not activated. The activity of important cell cycle regulatory genes was also examined in the liver following infection. There was increased expression of cyclin D1, cyclin E and cyclin A as well as proliferating cell nuclear antigen (PCNA) at 45, 60 and 215 days post infection. In addition, the levels of the cyclin-dependent kinase inhibitors p27(KIP1), p21(WAF1) and the tumor suppressor p53 were increased in the livers obtained from infected mice. Quantitative PCR revealed increased abundance of mRNA for cyclins A, D1 and E. Interestingly, cyclin A and E are ordinarily not found in the adult liver. Thus infection caused a reversion to a fetal/neonatal phenotype. These data provide a molecular basis for cell proliferation in the liver following T. cruzi infection.
Authors	Boumediene Bouzahzah, Fnu Nagajyothi, Mahalia S Desruisseaux, Mohan Krishnamachary, Stephen M Factor, Alex W Cohen, Michael P Lisanti, Stefka B Petkova, Richard G Pestell, Murray Wittner, Shankar Mukherjee, Louis M Weiss, Linda A Jelicks, Chris Albanese, Herbert B Tanowitz
Journal	Cell cycle (Georgetown, Tex.) (Cell Cycle) Vol. 5 Issue 20 Pg. 2396-400 (Oct 2006) ISSN: 1551-4005 [Electronic] United States
PMID	17102609 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
Chemical References	Cell Cycle Proteins Cyclin-Dependent Kinase Inhibitor Proteins Cyclins RNA, Messenger Tumor Suppressor Protein p53
Topics	Animals Cell Cycle Proteins (analysis, genetics) Cell Proliferation Chagas Disease Cyclin-Dependent Kinase Inhibitor Proteins (analysis) Cyclins (analysis, genetics) Liver (chemistry, parasitology, pathology) Mice Mice, Inbred Strains Phenotype RNA, Messenger (analysis) Trypanosoma cruzi Tumor Suppressor Protein p53 (analysis)

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