Fluorescent in situ hybridization for monosomy 3 via 30-gauge fine-needle aspiration biopsy of choroidal melanoma in vivo.

Abstract

OBJECTIVE:

To report the feasibility of intraoperative transscleral fine-needle aspiration biopsy at plaque surgery to obtain cells for monosomy 3 analysis in patients with choroidal melanoma.

DESIGN:

Consecutive interventional case series.

PARTICIPANTS:

Eighteen patients (18 eyes) with choroidal melanoma who had fine-needle aspiration biopsy performed with a 30-gauge needle at time of iodine 125 plaque placement.

INTERVENTION:

Cytology and cytogenetic analysis for monosomy 3 were obtained from biopsy specimens.

MAIN OUTCOME MEASURES:

Cytology, cytogenetic analysis for monosomy 3, and complications and feasibility of transscleral fine-needle aspiration biopsy of choroidal melanoma in vivo.

RESULTS:

Fine-needle aspiration biopsy was diagnostic of choroidal melanoma in 14 of 18 cases and resulted in viable cell cultures for fluorescent in situ hybridization (FISH) analysis in 9 cases. Fluorescent in situ hybridization for monosomy 3 was positive in 4 of the 9 cases. One patient had a mild vitreous hemorrhage. Tumors between 2 and 3 mm in height and those that yielded cells that did not attach in culture were most likely to have insufficient growth for FISH analysis.

CONCLUSIONS:

Transscleral fine-needle aspiration biopsy and FISH for monosomy 3 may provide important prognostic information on patients who undergo plaque radiotherapy for choroidal melanoma.

• Authors: Tara A Young, Nagesh P Rao, Ben J Glasgow, Joel N Moral, Bradley R Straatsma
• Journal: Ophthalmology (Ophthalmology) Vol. 114 Issue 1 Pg. 142-6 (Jan 2007) ISSN: 1549-4713 [Electronic] United States
• PMID: 17097737 (Publication Type: Journal Article)

Topics

• Adult
• Aged
• Aged, 80 and over
• Biopsy, Fine-Needle
• Brachytherapy
• Choroid Neoplasms (genetics, pathology, radiotherapy)
• Chromosomes, Human, Pair 3 (genetics)
• Cytogenetics (methods)
• Female
• Humans
• In Situ Hybridization, Fluorescence
• Male
• Melanoma (genetics, pathology, radiotherapy)
• Middle Aged
• Monosomy (genetics)