Histomorphological changes in murine fibrosarcoma after hypericin-based photodynamic therapy.

Abstract

Histomorphological changes in murine fibrosarcoma after photodynamic therapy (PDT) based on the natural photosensitizer hypericin were evaluated. C3H/DiSn mice were inoculated with fibrosarcoma G5:1:13 cells. When the tumour reached a volume of 40-80 mm(3) the mice were intraperitoneally injected with hypericin, either in a single dose (5 mg/kg; 1 or 6 h before laser irradiation) or two fractionated doses (2.5 mg/kg; 6 and 1 h before irradiation with laser light; 532 nm, 70 mW/cm(2), 168 J/cm(2)). All groups of PDT-treated animals with single and fractionated hypericin dosing presented primary vascular reactions including vascular dilatation, congestion, thrombosis and oedema. Two hours after PDT there were necrotic changes with small, rather focal appearance. One day after therapy the necrotic areas were enhanced, often affecting a complete superficial layer of tumour tissue. Necrotic areas were accompanied with inflammation and haemorrhages.

• Authors: Nikita Bobrov, Ivan Cavarga, Frantisek Longauer, Silvia Rybárová, Peter Fedorocko, Peter Brezáni, Pavol Miskovský, Ladislav Mirossay, Ján Stubna
• Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (Phytomedicine) Vol. 14 Issue 2-3 Pg. 172-8 (Feb 2007) ISSN: 0944-7113 [Print] Germany
• PMID: 17095201 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anthracenes
• Antineoplastic Agents, Phytogenic
• Photosensitizing Agents
• Plant Extracts
• Perylene
• hypericin

Topics

• Animals
• Anthracenes
• Antineoplastic Agents, Phytogenic (administration & dosage, pharmacology, therapeutic use)
• Cell Line, Tumor (drug effects)
• Disease Models, Animal
• Fibrosarcoma (drug therapy, pathology)
• Humans
• Hypericum
• Injections, Intraperitoneal
• Male
• Mice
• Mice, Inbred C3H
• Perylene (administration & dosage, analogs & derivatives, pharmacology, therapeutic use)
• Photochemotherapy
• Photosensitizing Agents (administration & dosage, pharmacology, therapeutic use)
• Phytotherapy
• Plant Extracts (administration & dosage, pharmacology, therapeutic use)
• Radiation Dosage