This study was aimed at determining the effects of
FrPbAII (174 Da), a novel isolated component from Parawixia bistriata
spider venom, in the CNS of Wistar rats. Considering that
FrPbAII inhibits the high affinity GABAergic uptake in a dose-dependent manner, its
anxiolytic and
anticonvulsant effects were analyzed in well-established animal models. Injection of
FrPbAII in the rat hippocampus induced a marked
anxiolytic effect, increasing the occupancy in the open arms of the elevated plus maze (EC(50)=0.09 microg/microl) and increasing the time spent in the lit area of the light-dark apparatus (EC(50)=0.03 microg/microl).
Anxiolytic effects were also observed considering the number of entries in the open arms of the EPM and in the lit compartment of the light-dark box. Interestingly, when microinjected bilaterally in the SNPr of freely moving rats,
FrPbAII (0.6 microg/microl) effectively prevented
seizures induced by the unilateral GABAergic blockade of Area tempestas (
bicuculline, 0.75 microg/microl). This
anticonvulsant effect was similar to that evoked by
muscimol (0.1 microg/microl) and
baclofen (0.6 microg/microl), but differed from that of the specific GAT1 inhibitor,
nipecotic acid (0.7 microg/microl). This difference could be accounted either for the parallel action of
FrPbAII over glycinergic transporters or to an inspecific activity on GABAergic transporters. Data from the present investigation might be pointing to a novel compound with interesting and yet unexplored pharmacological potential.