Human prolactin promotes human secondary immunoglobulin response in human/SCID mouse chimeras.

Abstract

Recombinant human prolactin (rhPRL) was administered to huPBL-SCID mice to determine its effects on production of human immunoglobulin (Ig). The huPBL-SCID mice were injected intraperitoneally (i.p.) with 10 mug rhPRL every other day for a total of 10 injections. The results reconfirmed that rhPRL significantly increased the numbers of human CD3+ T cells and human CD19+ B cells in spleens, lymph nodes, and thymuses of huPBL-SCID mice. The huPBL-SCID mice were then concurrently given various doses of diphtheria-tetanus (DT) vaccine and 10-mug i.p. injections of rhPRL and were examined for the presence of human DT-specific proliferation of lymph node cells in vitro and antibody production in vivo. rhPRL greatly improved the engraftment of functional human lymphocytes (CD3+ T cells and CD19+ B cells) in DT-immunized huPBL-SCID mice. The rhPRL-treated, DT-immunized huPBL-SCID mice produced significantly larger amounts of DT-specific antibodies in response to the vaccine. The predominant Ig isotype induced after immunization was IgG. Thus, rhPRL stimulation promotes human secondary IgG responses in huPBL-SCID mice.

• Authors: Jian Zhang, Rui Sun, Zhigang Tian
• Journal: Clinical and vaccine immunology : CVI (Clin Vaccine Immunol) Vol. 14 Issue 1 Pg. 60-4 (Jan 2007) ISSN: 1556-6811 [Print] United States
• PMID: 17079435 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Immunoglobulin G
• Immunoglobulins
• Recombinant Proteins
• Prolactin

Topics

• Animals
• Cytotoxicity, Immunologic (drug effects)
• Humans
• Immune System (drug effects, physiology)
• Immunoglobulin G (immunology)
• Immunoglobulins (immunology)
• Lymphocyte Activation
• Mice
• Mice, SCID
• Prolactin (pharmacology)
• Recombinant Proteins (genetics, metabolism)