| Abstract | Recombinant human prolactin (rhPRL) was administered to huPBL-SCID mice to determine its effects on production of human immunoglobulin (Ig). The huPBL-SCID mice were injected intraperitoneally (i.p.) with 10 mug rhPRL every other day for a total of 10 injections. The results reconfirmed that rhPRL significantly increased the numbers of human CD3+ T cells and human CD19+ B cells in spleens, lymph nodes, and thymuses of huPBL-SCID mice. The huPBL-SCID mice were then concurrently given various doses of diphtheria-tetanus (DT) vaccine and 10-mug i.p. injections of rhPRL and were examined for the presence of human DT-specific proliferation of lymph node cells in vitro and antibody production in vivo. rhPRL greatly improved the engraftment of functional human lymphocytes (CD3+ T cells and CD19+ B cells) in DT-immunized huPBL-SCID mice. The rhPRL-treated, DT-immunized huPBL-SCID mice produced significantly larger amounts of DT-specific antibodies in response to the vaccine. The predominant Ig isotype induced after immunization was IgG. Thus, rhPRL stimulation promotes human secondary IgG responses in huPBL-SCID mice. |
| Authors | Jian Zhang, Rui Sun, Zhigang Tian
(Affiliation: Institute of Immunopharmacology & Immunotherapy, School of Pharmaceutical Sciences, Shandong University, 44 Wenhua West Road, Jinan 250012, China. zhangj65 at sdu.edu.cn)
|
| Journal | Clinical and vaccine immunology : CVI
(Clin Vaccine Immunol)
Vol. 14
Issue 1
Pg. 60-4
(Jan 2007)
ISSN: 1556-6811 United States |
| PMID | 17079435
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Immunoglobulin G
- Immunoglobulins
- Recombinant Proteins
- Prolactin
|
| Topics |
- Animals
- Cytotoxicity, Immunologic
(drug effects)
- Humans
- Immune System
(drug effects, physiology)
- Immunoglobulin G
(immunology)
- Immunoglobulins
(immunology)
- Lymphocyte Activation
- Mice
- Mice, SCID
- Prolactin
(pharmacology)
- Recombinant Proteins
(genetics, metabolism)
|
