Abstract |
Dykellic acid, a novel factor initially identified from the culture broth of Westerdykella multispora F50733, has been shown to inhibit matrix metalloprotease 9 activity, caspase-3 activity, B cell proliferation and LPS-induced IgM production, suggesting that this factor may have anti- cancer effects. In an effort to further address the possible anti-tumoral effects of dykellic acid, we used wound healing, invasion and RhoA- GTP assays to examine the effects of dykellic acid on cell migration, invasion and angiogenesis. Our results revealed that dykellic acid dose-dependently inhibits B16 cell migration and motility, and inhibits HUVEC tube formation. Western blot analysis of the active form of RhoA (RhoA- GTP) showed that dykellic acid treatment decreased the levels of RhoA- GTP. These findings collectively suggest that dykellic acid may have both anti-metastatic and anti-angiogenic acitivites, and provides the first evidence for the involvement of RhoA in dykellic acid-induced effects.
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Authors | Jin-Chul Heo, Ja-Young Park, Sang-Uk Woo, Jae-Rang Rho, Ho-Jae Lee, Sung-Uk Kim, Yung-Hee Kho, Sang-Han Lee |
Journal | Biological & pharmaceutical bulletin
(Biol Pharm Bull)
Vol. 29
Issue 11
Pg. 2256-9
(Nov 2006)
ISSN: 0918-6158 [Print] Japan |
PMID | 17077524
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Propionates
- Pyrones
- dykellic acid
- rhoA GTP-Binding Protein
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Topics |
- Animals
- Blood Vessels
(drug effects, physiology)
- Blotting, Western
- Cell Line
- Cell Line, Tumor
- Cell Movement
(drug effects)
- Cell Proliferation
(drug effects)
- Dose-Response Relationship, Drug
- Endothelial Cells
(drug effects, physiology)
- Humans
- Mice
- Neoplasm Invasiveness
(prevention & control)
- Neovascularization, Pathologic
(metabolism, prevention & control)
- Propionates
(chemistry, pharmacology)
- Pyrones
(chemistry, pharmacology)
- rhoA GTP-Binding Protein
(metabolism)
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