2-methoxycinnamaldehyde reduces IL-1beta-induced prostaglandin production in rat cerebral endothelial cells.

Abstract

Prostaglandin E2 (PGE2) works as a common final mediator of the febrile. Guizhi-Tang, one of the most famous traditional Chinese medical formula used to treat influenza, common cold and other pyretic conditions, was previously reported to reduce the production of PGE 2 in rats. 2-Methoxycinnamaldehyde is a principle compound isolated from Guizhi-Tang. The aim of the present study was to investigate the effects of 2-methoxycinnamaldehyde on PGE2 production of rat cerebral endothelial cells (CECs). 2-Methoxycinnamaldehyde dose-dependently inhibited interleukin (IL)-1beta-induced PGE2 production in CECs with IC50 values of 174 microM. IL-1beta stimulation increased the protein, activity and mRNA expression of cyclooxygenase (COX)-2 but not COX-1. 2-Methoxycinnamaldehyde reduced IL-1beta-induced protein and activity of COX-2, but did not influence the COX-2 mRNA expression. Our results show that prostaglandin production in CECs during stimulated conditions is sensitive to inhibition by 2-methoxycinnamaldehyde and suggest that 2-methoxycinnamaldehyde may reduce COX-2 protein level and activity but not COX-2 mRNA.

• Authors: Jian-You Guo, Hai-Ru Huo, Yuan-Xiao Yang, Cang-Hai Li, Hong-Bin Liu, Bao-Sheng Zhao, Lan-Fang Li, Yue-Ying Ma, Shu-Ying Guo, Ting-Liang Jiang
• Journal: Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 29 Issue 11 Pg. 2214-21 (Nov 2006) ISSN: 0918-6158 [Print] Japan
• PMID: 17077517 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Analgesics, Non-Narcotic
• Cinnamates
• Drugs, Chinese Herbal
• Interleukin-1beta
• Membrane Proteins
• RNA, Messenger
• von Willebrand Factor
• 2-methoxycinnamaldehyde
• Acrolein
• Cyclooxygenase 2
• PTGS2 protein, human
• Dinoprostone

Topics

• Acrolein (analogs & derivatives, chemistry, isolation & purification, pharmacology)
• Analgesics, Non-Narcotic (chemistry, isolation & purification, pharmacology)
• Animals
• Blotting, Western
• Cells, Cultured
• Cerebral Cortex (blood supply)
• Cinnamates (chemistry, isolation & purification, pharmacology)
• Cyclooxygenase 2 (genetics, metabolism)
• Dinoprostone (biosynthesis)
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (chemistry, isolation & purification, pharmacology)
• Endothelium, Vascular (cytology, drug effects, metabolism)
• Enzyme-Linked Immunosorbent Assay
• Interleukin-1beta (pharmacology)
• Membrane Proteins (antagonists & inhibitors, genetics, metabolism)
• RNA, Messenger (genetics, metabolism)
• Rats
• Reverse Transcriptase Polymerase Chain Reaction
• von Willebrand Factor (analysis)