[Study of the humoral immune suppression of recombinant human collagen type II peptide 250-270].

Abstract

AIM:

To investigate the modulation of rhC II 250-270 peptide on special humoral immune response in the course of oral administration.

METHODS:

ELISA was used to determine antigen-specific antibodies in mice sera. The frequency of anti-C II and anti-C II (250-270) antibody-forming spleen cells was measured by ELISPOT.

RESULTS:

The level of C II- and C II (250-270)- specific IgG in serum from the mice fed with rhC II (250-270) were (0.82+/-0.02) and (0.84+/-0.04) respectively, and lower significantly than those of collagen-induced arthritis (CIA) control group. The anti-C II (250-270) antibody responses were suppressed obviously (P<0.01). The frequency of antibody-forming cells in the spleen from rhC II (250-270)-fed mice were (158+/-9 counts/well) and (181+/-10 counts/well) respectively, and also were reduced significantly when compared with that in CIA control group (247+/-16 counts/well)(P<0.05).

CONCLUSION:

Oral rhC II (250-270) could induce specific suppression of humoral responds in CIA. These findings together with a better understanding of the mechanisms of oral tolerance and regulation of humoral immune response in CIA, will help the development of innovative therapeutic intervention for rheumatoid arthritis.

• Authors: Hong-Kun Wang, Ping Zhu, Xiao-Yan Li, Zhao-Hui Zheng, Jun-Feng Jia, Jin Ding, Chun-Mei Fan
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 22 Issue 6 Pg. 742-4 (Nov 2006) ISSN: 1007-8738 [Print] China
• PMID: 17077014 (Publication Type: Journal Article)

Chemical References

• Collagen Type II
• Peptide Fragments
• Recombinant Proteins

Topics

• Animals
• Antibody Formation (drug effects)
• Antibody Specificity
• Arthritis, Experimental (immunology, pathology)
• Arthritis, Rheumatoid (immunology, therapy)
• Collagen Type II (chemistry, pharmacology)
• Humans
• Immune Tolerance (drug effects)
• Immunosuppression Therapy
• Male
• Mice
• Peptide Fragments (administration & dosage, chemistry, pharmacology)
• Recombinant Proteins (administration & dosage, chemistry, pharmacology)
• Spleen (immunology, pathology)