HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Pannarin inhibits cell growth and induces cell death in human prostate carcinoma DU-145 cells.

Abstract
In the course of our continuing search for new natural anticancer compounds for treatment and/or prevention of prostate cancer, our laboratory has focused its search on poorly investigated lichen metabolites, sphaerophorin, pannarin and epiphorellic acid-1. To this end, we treated DU-145, a cell line resembling the last stage of prostate carcinoma, with different concentrations (6-50 micromol/l) of these compounds for 72 h. Our data clearly evidenced that these lichen metabolites inhibit the growth of human prostate carcinoma DU-145 cells, but pannarin exhibits a higher effect. Our data show an induction of apoptotic death of advanced prostate cancer cells by sphaerophorin, pannarin and epiphorellic acid-1. In fact, a significant (P<0.001) increase in caspase-3 enzyme activity occurred in DU-145 cells treated with all lichen compounds at 12 and 25 micromol/l concentrations, correlated to a high DNA fragmentation, but without the disruption of the plasma membrane, as evaluated by the percentage of lactic dehydrogenase release. Alternatively, we found a low, but significant (P<0.01) lactic dehydrogenase release at higher concentrations (50 micromol/l), suggesting that in these experimental conditions sphaerophorin, pannarin and epiphorellic acid-1 induce necrosis in DU-145 cells, through the increase in reactive oxygen species generation. The experimental evidence is further confirmed by caspase-3 activity results, evidencing a reduction in the activity of this protease at a higher concentration, 50 micromol/l.
AuthorsAlessandra Russo, Marisa Piovano, Laura Lombardo, Luca Vanella, Venera Cardile, Juan Garbarino
JournalAnti-cancer drugs (Anticancer Drugs) Vol. 17 Issue 10 Pg. 1163-9 (Nov 2006) ISSN: 0959-4973 [Print] England
PMID17075315 (Publication Type: Evaluation Study, Journal Article)
Chemical References
  • Antineoplastic Agents, Phytogenic
  • Benzoates
  • Benzoxepins
  • Depsides
  • Reactive Oxygen Species
  • epiphorellic acid-1
  • sphaerophorin
  • pannarin
  • L-Lactate Dehydrogenase
  • Caspase 3
Topics
  • Antineoplastic Agents, Phytogenic (pharmacology)
  • Apoptosis (drug effects)
  • Benzoates (pharmacology)
  • Benzoxepins (pharmacology)
  • Carcinoma (metabolism, pathology)
  • Caspase 3 (metabolism)
  • Cell Death (drug effects)
  • Cell Growth Processes (drug effects)
  • Cell Survival (drug effects)
  • DNA Fragmentation (drug effects)
  • Depsides (pharmacology)
  • Humans
  • L-Lactate Dehydrogenase (metabolism)
  • Lichens (chemistry)
  • Male
  • Models, Biological
  • Prostatic Neoplasms (metabolism, pathology)
  • Reactive Oxygen Species (metabolism)
  • Tumor Cells, Cultured

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: