[The significance of DNA-binding inhibitor 4 promoter methylation status in acute leukemia].

Abstract

OBJECTIVE:

To investigate the relationship between promoter methylation of inhibitor of DNA binding 4 (Id4) gene and acute leukemia.

METHODS:

We detected the status of promoter methylation of Id4 gene in peripheral blood, peripheral blood stem cell transplantation (PBSC) and bone marrow samples from health donors, bone marrow cells from leukemia patients and leukemia cell lines with MS-PCR methods.

RESULTS:

The frequency of Id4 promoter methylation was 21/25 in acute myeloid leukemia (84%), 12/14 in acute lymphoblastic leukemia (86%), 8/8 in relapse acute leukemias and 2/2 in leukemia cell lines whereas no methylation was detected in peripheral blood, PBSC and bone marrow samples from healthy donors. In addition, Hek937 cell line was unmethylated.

CONCLUSION:

Id4 promoter methylation was detected only in acute leukemia patients and leukemia cell lines and it might play a role in leukemia genesis.

• Authors: Yu Zhao, Li Yu, Quan-shun Wang, Hong-hua Li, Jian Bo, Li-ping Dou
• Journal: Zhonghua nei ke za zhi (Zhonghua Nei Ke Za Zhi) Vol. 45 Issue 7 Pg. 576-8 (Jul 2006) ISSN: 0578-1426 [Print] China
• PMID: 17074115 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ID4 protein, human
• Inhibitor of Differentiation Proteins

Topics

• DNA Methylation
• Humans
• Inhibitor of Differentiation Proteins (genetics, metabolism)
• K562 Cells (metabolism)
• Leukemia, Myeloid, Acute (genetics, metabolism)
• Polymerase Chain Reaction
• Precursor Cell Lymphoblastic Leukemia-Lymphoma (genetics, metabolism)
• Promoter Regions, Genetic
• U937 Cells (metabolism)