Increased lipid peroxidation in patients with non-alcoholic fatty liver disease and chronic hepatitis C as measured by the plasma level of 8-isoprostane.

Abstract

BACKGROUND:

Oxidative stress plays an important role in the pathogenesis of chronic liver diseases. The plasma level of 8-isoprostane, a product of lipid peroxidation, is a marker of oxidative stress in vivo. The aim of the present study was to clarify whether the degree of lipid peroxidation, as measured by the plasma level of 8-isoprostane, influences the progression of chronic liver diseases and hepatocarcinogenesis.

METHODS:

Plasma 8-isoprostane levels were investigated in 14 patients with non-alcoholic fatty liver disease (NAFLD), 75 with chronic hepatitis C (CH-C), 14 with cured CH-C, 14 with HCV-positive hepatocellular carcinoma (HCC-C) and 38 healthy volunteers. 8-Isoprostane was measured by enzyme immunoassay after affinity column purification.

RESULTS:

Plasma 8-isoprostane was significantly elevated in NAFLD (11.9 [3.8-56.8] pg/mL), CH-C (10.1 [4.2-134.5] pg/mL) as compared to controls (6.3 [3.6-11.1] pg/mL). Plasma 8-isoprostane values were positively correlated with body mass index in NAFLD (P < 0.05) and with total cholesterol in cured CH-C (P < 0.01). 8-Isoprostane levels were not significantly related to sex, age, biochemical data or iron metabolism markers in all liver diseases. In addition, after the administration of peg-interferon, the values of 8-isoprostane improved in almost all patients, reaching values of healthy subjects.

CONCLUSIONS:

8-Isoprostane values are elevated in patients with NAFLD and CH-C as compared to healthy controls. Oxidative stress caused by increased lipid peroxidation is involved in the pathogenesis of NAFLD and CH-C.

• Authors: Masayoshi Konishi, Motoh Iwasa, Jun Araki, Yoshinao Kobayashi, Akira Katsuki, Yasuhiro Sumida, Naoki Nakagawa, Yuji Kojima, Shozo Watanabe, Yukihiko Adachi, Masahiko Kaito
• Journal: Journal of gastroenterology and hepatology (J Gastroenterol Hepatol) Vol. 21 Issue 12 Pg. 1821-5 (Dec 2006) ISSN: 0815-9319 [Print] Australia
• PMID: 17074020 (Publication Type: Journal Article)

Chemical References

• Antiviral Agents
• Biomarkers
• Drug Carriers
• Interferon alpha-2
• Interferon-alpha
• Recombinant Proteins
• 8-epi-prostaglandin F2alpha
• Polyethylene Glycols
• Dinoprost
• peginterferon alfa-2a

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Antiviral Agents (therapeutic use)
• Biomarkers (blood)
• Dinoprost (analogs & derivatives, blood)
• Disease Progression
• Drug Carriers
• Fatty Liver (blood, pathology)
• Female
• Hepatitis C, Chronic (blood, drug therapy, pathology)
• Humans
• Immunoenzyme Techniques
• Interferon alpha-2
• Interferon-alpha (therapeutic use)
• Lipid Peroxidation (physiology)
• Male
• Middle Aged
• Polyethylene Glycols (therapeutic use)
• Prognosis
• Recombinant Proteins