Further studies on galanin-, substance P-, and CGRP-like immunoreactivities in primary sensory neurons and spinal cord: effects of dorsal rhizotomies and sciatic nerve lesions.

Abstract

The peptides galanin (GAL), substance P (SP), and calcitonin gene-related peptide (CGRP) were analyzed with immunohistochemistry and radioimmunoassay in the spinal cord, dorsal root ganglia, dorsal roots, and sciatic nerve of normal rats and rats subjected to several experimental procedures, including ligation, crush, and/or sectioning of nerves. The results show that peripheral nerve transection induces a dramatic increase in GAL content both in dorsal roots and sciatic nerve, demonstrating that this lesion causes an increased out-transport of the newly synthesized peptide both into the central and peripheral branches of the primary sensory neurons. In contrast evidence was obtained for decreased out-transport of SP and CGRP. The functional significance of these findings remains to be analyzed.

• Authors: M J Villar, Z Wiesenfeld-Hallin, X J Xu, E Theodorsson, P C Emson, T Hökfelt
• Journal: Experimental neurology (Exp Neurol) Vol. 112 Issue 1 Pg. 29-39 (Apr 1991) ISSN: 0014-4886 [Print] United States
• PMID: 1707368 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Neuropeptides
• Peptides
• Substance P
• Galanin
• Calcitonin Gene-Related Peptide

Topics

• Animals
• Calcitonin Gene-Related Peptide (analysis)
• Fluorescent Antibody Technique
• Galanin
• Ganglia, Spinal (cytology, physiology)
• Male
• Nerve Crush
• Neurons, Afferent (cytology, physiology)
• Neuropeptides (analysis)
• Peptides (analysis)
• Radioimmunoassay
• Rats
• Rats, Inbred Strains
• Reference Values
• Sciatic Nerve (cytology, physiology)
• Spinal Cord (cytology, physiology)
• Spinal Nerves (physiology)
• Substance P (analysis)