Expression and distribution of Gpr119 in the pancreatic islets of mice and rats: predominant localization in pancreatic polypeptide-secreting PP-cells.

Abstract	The GPR119 was recently shown to be activated by oleoylethanolamide (OEA), a naturally occurring bioactive lipid with hypophagic and anti-obesity effects. In this study, we have cloned and characterized its murine counterpart, Gpr119. The full-length cDNA contained an open reading frame of 1008bp encoding a 335-amino acid protein. The genomic organization of Gpr119 was unique, having a 3'-untranslated second exon that was also involved in an alternative splicing event. Gene expression analyses confirmed its specific expressions in pancreatic islets and two endocrine cell-lines, MIN6 and alphaTC1. Immunohistochemistry and double-immunofluorescence studies using a specific antibody revealed the predominant Gpr119 localization in pancreatic polypeptide (PP)-cells of islets. No definitive evidence of Gpr119-immunoreactivity in adult beta- or alpha-cells was obtained. The Gpr119 mRNA levels were elevated in islets of obese hyperglycemic db/db mice as compared to control islets, suggesting a possible involvement of this receptor in the development of obesity and diabetes.
Authors	Yukiko Sakamoto, Hiroshi Inoue, Shuhei Kawakami, Katsuyuki Miyawaki, Tatsuro Miyamoto, Kuniko Mizuta, Mitsuo Itakura
Journal	Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 351 Issue 2 Pg. 474-80 (Dec 15 2006) ISSN: 0006-291X [Print] United States
PMID	17070774 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	3' Untranslated Regions Gpr119 protein, mouse RNA, Messenger Receptors, G-Protein-Coupled Recombinant Fusion Proteins
Topics	3' Untranslated Regions Alternative Splicing Animals Base Sequence Cell Line Cloning, Molecular Exons Hyperglycemia (metabolism) Islets of Langerhans (metabolism) Male Mice Mice, Obese Molecular Sequence Data Open Reading Frames Pancreatic Polypeptide-Secreting Cells (metabolism) RNA, Messenger (metabolism) Rats Receptors, G-Protein-Coupled (genetics, metabolism) Recombinant Fusion Proteins (genetics, metabolism)

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