[Over-expression of Hypoxia-inducible factor 1alpha increases invasive potency of LNCaP cells in vitro].

Abstract

OBJECTIVE:

To evaluate the effect of hypoxia-inducible factor-1alpha (HIF1alpha) over-expression on the invasive potency of human prostate cancer cell.

METHODS:

Human prostate cancer cells of the line LNCaP were cultured and transfected by the recombinant plasmid pcDNA3.1(-)-HIF-1alpha containing the gene HIF1alpha with Lipofectamine 2000 system. The positive clone cells were selected by G418 and confirmed by Western blotting and immunofluorescence staining (LNCaP/HIF1alpha cells). Transwell chambers with polycarbonate filter were coated by 100 microl Matrigel at 1:20 dilution in serum-free medium. LNCaP cell suspension and LNCaP/HIF1alpha cell suspension were inoculated into the Transwell chambers respectively for 24 hours to analyze the invasive potency. Western blotting was used to detect the expression of E-cadherin, vimentin, matrix metalloproteinase-2 (MMP-2), cathepsin D, and urokinase-type plasminogen activator receptor (uPAR).

RESULTS:

The expression level of HIF1alpha in the LNCaP/HIF1alpha cells was distinctly higher than that in the LNCaP cells. The numbers of LNCaP-HIF1alpha cells penetrating through the Transwell polycarbonate filter was 4.6 +/- 0.4 x 10(4), significantly higher than that of the LNCaP cells (3.2 +/- 0.3 x 10(4), P < 0.01). The expressions of vimentin, MMP-2, cathepsin D, and uPAR were all up-regulated in LNCaP-HIF1alpha cells than those of the LNCaP cells. Whereas, the expression of E-cadherin was down-regulated in the LNCaP-HIF1alpha cells.

CONCLUSION:

Over-expression of HIF-1alpha stimulates the invasion potency of human prostate carcinoma cell. The expression of E-cadherin, vimentin, MMP-2, cathepsin D, and uPAR, all playing an established role in the invasion of tumor, can be regulated by HIF-1 in human prostate cancer cell.

• Authors: Yong Luo, Da-lin He, Liang Ning, Shu-lin Shen, Lei Li, Xiang Li
• Journal: Zhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 86 Issue 32 Pg. 2285-8 (Aug 29 2006) ISSN: 0376-2491 [Print] China
• PMID: 17064578 (Publication Type: English Abstract, Journal Article)

Chemical References

• Hypoxia-Inducible Factor 1, alpha Subunit

Topics

• Cell Line, Tumor
• Humans
• Hypoxia-Inducible Factor 1, alpha Subunit (biosynthesis)
• Male
• Neoplasm Invasiveness
• Prostatic Neoplasms (metabolism, pathology)