Biological role of estrogen receptor beta in salivary gland adenocarcinoma cells.

Abstract

PURPOSE:

This study is intended to investigate the biological role of estrogen receptor (ER) nongenomic signaling in salivary gland adenocarcinoma cells that predominantly express ERbeta.

EXPERIMENTAL DESIGN:

Salivary gland adenocarcinoma cell lines HSG and HSY were used to study the effect of diarylpropionitrile and estrogen on the nongenomic signaling of ERbeta, cytoskeletal remodeling, and cell motility.

RESULTS:

We found that diarylpropionitrile and estrogen triggered rapid activation of the extracellular signal-regulated kinase 1/2 (ERK), Src, and focal adhesion kinase signaling pathways. Estrogen stimulation also induced long cytoplasmic extensions, filopodia formation, and abnormal outgrowths in both HSG and HSY cells. We further observed that ligand-induced migration of these cells was blocked by the pure antiestrogen ICI 182780 and the mitogen-activated protein/ERK kinase inhibitor PD98059, indicating that estrogen-induced cell migration is mediated by the activation of ERbeta nongenomic signaling.

CONCLUSION:

These results clearly showed that ERbeta nongenomic signaling is active in salivary gland cells and has a biological role in migration, presumably via the stimulation of ERK1/2. In future, the findings of this study might have clinical importance as several ERbeta-selective agonists are currently being available, and these could potentially be used for therapeutic targeting of ERbeta-positive salivary tumors.

• Authors: Kazufumi Ohshiro, Suresh K Rayala, Michelle D Williams, Rakesh Kumar, Adel K El-Naggar
• Journal: Clinical cancer research : an official journal of the American Association for Cancer Research (Clin Cancer Res) Vol. 12 Issue 20 Pt 1 Pg. 5994-9 (Oct 15 2006) ISSN: 1078-0432 [Print] United States
• PMID: 17062671 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Estrogen Receptor alpha
• Estrogen Receptor beta
• Mitogen-Activated Protein Kinases

Topics

• Adenocarcinoma (physiopathology)
• Cell Line, Tumor
• Cell Movement
• Estrogen Receptor alpha (physiology)
• Estrogen Receptor beta (physiology)
• Humans
• Mitogen-Activated Protein Kinases (metabolism)
• Salivary Gland Neoplasms (physiopathology)
• Signal Transduction
• Wound Healing