Experimental study on in vitro tumor cell killing by focused bi-frequency ultrasound activated hematoporphyrin derivatives.

Abstract

OBJECTIVE:

As ultrasound has become an important therapeutic technique on tumor, in order to find the cell-killing effect of focused bi-frequency ultrasound activated hematoporphyrin derivatives, experimental study was processed in vitro.

METHODS:

Tumor cells K562 and SW-480 were killed by focused bi-frequency ultrasound activated hematoporphyrin (Hp) derivatives under optimized ultrasonic parameters and the cell-killing effect was tested by MTT (Thiazolyl blue: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) technique.

RESULTS:

The lower frequency (0.6 MHz) was better than the higher (1.1 MHz) in activating hematoporphyrin derivatives; the bi-frequency was better than mono-frequency, and the cell-killing efficiency was 2-3 times of sum of the two kinds of ultrasounds which constitute bi-frequency. Incubation for the 16 h was better than 4 h after the cells exposed to the ultrasound.

CONCLUSION:

The method of killing tumor cells by focused bi-frequency ultrasound activated hematoporphyrin derivatives is presented and evaluated in this paper.

• Authors: Z Shang, X Ren, J Zhang, X Zhu, H Qi, Q Liu, K Zhang, S Wu, Y Zeng
• Journal: Australasian physical & engineering sciences in medicine (Australas Phys Eng Sci Med) Vol. 29 Issue 3 Pg. 267-71 (Sep 2006) ISSN: 0158-9938 [Print] Netherlands
• PMID: 17058589 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Technical Report)

Chemical References

• Hematoporphyrins
• Photosensitizing Agents

Topics

• Apoptosis (drug effects)
• Cell Line, Tumor
• Colonic Neoplasms (drug therapy, pathology)
• Hematoporphyrins (therapeutic use)
• Humans
• K562 Cells
• Photosensitizing Agents (therapeutic use)
• Treatment Outcome
• Ultrasonic Therapy (methods)