Abstract |
Due to its highly parallel approach, DNA microarray technology opens up new possibilities that may be particularly beneficial for laboratory diagnosis of infectious diseases. We developed a microarray assay for detection and differentiation of all currently defined chlamydial species belonging to the genera Chlamydia and Chlamydophila using the ArrayTube system, which we found to be particularly user-friendly and economical. The test includes PCR amplification of a 23S rDNA target region with concurrent biotinylation and subsequent hybridisation in the ArrayTube, a micro-reaction tube carrying the microarray chip on the bottom. In addition to high specificity, the assay was shown to allow detection and genetic characterisation of single PCR-amplifiable target DNA copies.
|
Authors | K Sachse, H Hotzel, P Slickers, R Ehricht |
Journal | Developments in biologicals
(Dev Biol (Basel))
Vol. 126
Pg. 203-10; discussion 326-7
( 2006)
ISSN: 1424-6074 [Print] Switzerland |
PMID | 17058496
(Publication Type: Journal Article)
|
Topics |
- Chlamydia
(classification, genetics, isolation & purification)
- Chlamydia Infections
(diagnosis, microbiology)
- Chlamydophila
(classification, genetics, isolation & purification)
- Chlamydophila Infections
(diagnosis, microbiology)
- Humans
- Oligonucleotide Array Sequence Analysis
(methods)
- Reproducibility of Results
- Sensitivity and Specificity
- Species Specificity
|