Abstract |
Reactive oxygen species (ROS) generated by ultraviolet (UV) irradiation are counterbalanced by endogenous antioxidant systems. To test the hypothesis of a novel photoprotective approach, we irradiated epidermis reconstructed with normal human keratinocytes overexpressing sustainably lentivirus-mediated catalase (CAT), copper/ zinc superoxide dismutase (CuZnSOD) or manganese superoxide dismutase (MnSOD) enzymes. We found that following UVB irradiation there was a marked decrease in sunburn cell formation, caspase-3 activation and p53 accumulation in human reconstructed epidermis overexpressing CAT. Moreover, UVA-induced hypertrophy and DNA oxidation (8-oxodeoxyguanosine) were decreased by CAT overexpression. These effects were not achieved by overexpression of CuZnSOD or MnSOD. In conclusion, vector-mediated CAT overexpression could be a promising photoprotective tool against deleterious effects of UV irradiation such skin cancer especially in monogenic/polygenic photosensitive disorders characterized by ROS accumulation.
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Authors | H R Rezvani, M Cario-André, C Pain, C Ged, H deVerneuil, A Taïeb |
Journal | Cancer gene therapy
(Cancer Gene Ther)
Vol. 14
Issue 2
Pg. 174-86
(Feb 2007)
ISSN: 0929-1903 [Print] England |
PMID | 17053817
(Publication Type: Journal Article)
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Chemical References |
- Catalase
- Superoxide Dismutase
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Topics |
- Apoptosis
- Catalase
(genetics, metabolism)
- Epidermis
(enzymology, radiation effects)
- Humans
- Immunohistochemistry
- Superoxide Dismutase
(genetics, metabolism)
- Transduction, Genetic
- Transgenes
- Ultraviolet Rays
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