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Characterization of Japanese flounder (Paralichthys olivaceus) NK-lysin, an antimicrobial peptide.

Abstract
The NK-lysin cDNA of Japanese flounder, Paralichthys olivaceus, consists of 657bp, containing an open reading frame (ORF) of 444bp, which encodes 147 amino acid residues. The amino acid sequence of Japanese flounder NK-lysin has 21% identity to porcine NK-lysin and bovine NK-lysin, 23% to equine NK-lysin, and 46% to zebrafish NK-lysin-like protein. Multiple alignments of Japanese flounder NK-lysin and other known saposin-like proteins revealed that the six cysteine residues important for structural folding are completely conserved. The Japanese flounder NK-lysin gene is approximately 2kb and consists of five exons and four introns. Japanese flounder NK-lysin mRNA constitutive expression was mainly detected in gills, heart, head kidney, intestines, peripheral blood leukocytes (PBLs), spleen and trunk kidney, and was detected at low levels in liver, muscle and ovary. However, expression was not detected in brain, skin and stomach of apparently healthy Japanese flounder. Gene expression of Japanese flounder NK-lysin was not inducible by lipopolysaccharide (LPS) treatment. A synthesized NK-lysin peptide, consisting of 27 amino acid residues, showed antimicrobial activity against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Photobacterium damselae subsp. piscicida.
AuthorsIkuo Hirono, Hidehiro Kondo, Takashi Koyama, Nur Rahmawaty Arma, Jee Youn Hwang, Reiko Nozaki, Naoko Midorikawa, Takashi Aoki
JournalFish & shellfish immunology (Fish Shellfish Immunol) Vol. 22 Issue 5 Pg. 567-75 (May 2007) ISSN: 1050-4648 [Print] England
PMID17046282 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • CD8 Antigens
  • DNA Primers
  • DNA, Complementary
  • Lipopolysaccharides
  • NK-lysin
  • Proteolipids
Topics
  • Amino Acid Sequence
  • Animals
  • Bacteria (drug effects)
  • Base Sequence
  • CD8 Antigens (biosynthesis)
  • DNA Primers (chemistry)
  • DNA, Complementary (chemistry)
  • Flounder (genetics, physiology)
  • Gene Expression (drug effects, immunology)
  • Gene Expression Profiling (veterinary)
  • Lipopolysaccharides (pharmacology)
  • Molecular Sequence Data
  • Proteolipids (biosynthesis, chemistry, genetics, pharmacology)
  • Sequence Alignment

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