Paraoxonase-1 (PON1) activity, but not PON1(Q192R) phenotype, is a predictor of coronary artery disease in a middle-aged Serbian population.

Abstract

BACKGROUND:

Paraoxonase-1 (PON1) is a high-density lipoprotein (HDL)-associated serum enzyme that protects lipoproteins from oxidative modifications. Polymorphisms in the gene, including PON1Q192R, have been studied. However, inconsistencies regarding the above-mentioned polymorphism obscure its association with vascular disease.

METHODS:

Using a two-substrate (paraoxon/diazoxon) activity method, we investigated the frequencies of PON1Q192R phenotypes in 261 middle-aged subjects: 156 patients with angiographically assessed coronary heart disease (CHD) and 105 CHD-free subjects as the control group. The PON1(192) phenotype was predicted from examination of the two-dimensional plot of hydrolysis rates of diazoxon vs. paraoxon and by using the antimode of the histogram of the ratio of diazoxonase/paraoxonase activity.

RESULTS:

The PON1Q192R phenotype frequencies in 113 patients with occlusion >50% (coronary artery disease-positive, CAD+ group) vs. control population were as follows: QQ (0.552 vs. 0.510), QR (0.382 vs. 0.408) and RR (0.066 vs. 0.082); chi2=0.414, p=0.813. We found lower paraoxonase (POase) and diazoxonase (DZOase) activities in the CAD+ patients when compared to the control population. According to logistic regression analysis, POase activity was a better predictor of coronary disease onset compared with DZOase activity measurements and PON1Q192R phenotyping.

CONCLUSIONS:

We conclude that enzyme activity (within a particular phenotypic group) is more important than phenotype alone in predicting susceptibility to coronary artery disease.

• Authors: Jelena Kotur-Stevuljevic, Slavica Spasic, Aleksandra Stefanovic, Aleksandra Zeljkovic, Natasa Bogavac-Stanojevic, Dimitra Kalimanovska-Ostric, Vesna Spasojevic-Kalimanovska, Zorana Jelic-Ivanovic
• Journal: Clinical chemistry and laboratory medicine (Clin Chem Lab Med) Vol. 44 Issue 10 Pg. 1206-13 ( 2006) ISSN: 1434-6621 [Print] Germany
• PMID: 17032132 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Aryldialkylphosphatase
• PON1 protein, human

Topics

• Aryldialkylphosphatase (blood, genetics)
• Coronary Artery Disease (enzymology, epidemiology, genetics)
• Female
• Humans
• Male
• Middle Aged
• Phenotype
• Polymorphism, Genetic
• Predictive Value of Tests
• Prognosis
• Yugoslavia (epidemiology)