Tumor antigen occurs in N-glycan of royal jelly glycoproteins: honeybee cells synthesize T-antigen unit in N-glycan moiety.

Abstract

In our previous paper (Kimura, Y., et al., Biosci. Biotechnol. Biochem., 67, 1852-1856, 2003), we found that a complex type N-glycans containing beta1-3 galactose residue occurs on royal jelly glycoproteins. During structural analysis of minor components of royal jelly N-glycans, we found complex type N-glycans bearing both galactose and N-acetylgalactosamine residues. Detailed structural analysis of pyridylaminated oligosaccharide revealed that the newly found N-glycan had a complex type structure harboring a tumor marker (T-antigen) unit: Galbeta1-3GalNAcbeta1-4GlcNAcbeta1-2Manalpha1-6 (Galbeta1-3GalNAcbeta1-4GlcNAcbeta1-2Manalpha1-3) Manbeta1-4GlcNAcbeta1-4GlcNAc. To our knowledge, this may be the first report of the presence of the T-antigen unit in the N-glycan moiety of eucaryotic glycoproteins.

• Authors: Yoshinobu Kimura, Takayuki Ushijima, Megumi Maeda, Yoichiro Hama, Mariko Kimura, Kiyoshi Okihara, Hiroyuki Sugimoto, Hideo Yamada
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 70 Issue 10 Pg. 2583-7 (Oct 2006) ISSN: 0916-8451 [Print] England
• PMID: 17031027 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Antigens, Viral, Tumor
• Fatty Acids
• Glycoproteins
• Peptide Fragments
• Polysaccharides
• royal jelly

Topics

• Animals
• Antigens, Neoplasm (chemistry, isolation & purification)
• Antigens, Viral, Tumor (biosynthesis)
• Bees (chemistry, immunology)
• Carbohydrate Sequence
• Chromatography, High Pressure Liquid
• Fatty Acids (chemistry)
• Glycoproteins (analysis, immunology)
• Molecular Sequence Data
• Peptide Fragments (biosynthesis)
• Polysaccharides (analysis, chemistry, immunology)
• Spectrometry, Mass, Electrospray Ionization