Proteome analysis of responses to ascochlorin in a human osteosarcoma cell line by 2-D gel electrophoresis and MALDI-TOF MS.

Abstract

Ascochlorin is a prenyl-phenol compound that was isolated from the fungus Ascochyta viciae. Ascochlorin reduces serum cholesterol and triglyceride levels, suppresses hypertension and tumor development, and ameliorates type I and II diabetes. Here, to better understand the mechanisms by which ascochlorin regulates physiological or pathological events and induces responses in the pharmacological treatment of cancer, we performed differential analysis of the proteome of the human osteosarcoma cells U2OS in response to ascochlorin. In addition, we established the first two-dimensional map of the U2OS proteome. The U2OS cell proteomes with and without treatment with ascochlorin were compared using two-dimensional electrophoresis, matrix-assisted laser desorption/ionization mass spectrometry and bioinformatics. The largest differences in expression were observed for the epidermal growth factor receptor (4-fold decrease), ribulose-5-phosphate-epimerase (13-fold decrease), ATP-dependent RNA helicase (8-fold decrease), and kelch-like ECH-associated protein 1 (6-fold decrease). The abundance of heterogeneous nuclear ribonucleoprotein L and minichromosome maintenance protein 7 increased 12- and 8.2-fold, respectively. In addition, Erk 2 was increased 3-fold in U2OS cells treated with ascochlorin. The expression of some selected proteins was confirmed by western blotting, zymography and RT-PCR analysis.

• Authors: Jeong Han Kang, Kwan-Kyu Park, In-Seon Lee, Junji Magae, Kunio Ando, Cheorl-Ho Kim, Young-Chae Chang
• Journal: Journal of proteome research (J Proteome Res) Vol. 5 Issue 10 Pg. 2620-31 (Oct 2006) ISSN: 1535-3893 [Print] United States
• PMID: 17022633 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alkenes
• Antineoplastic Agents
• Biomarkers, Tumor
• Neoplasm Proteins
• Phenols
• Proteome
• ascochlorin

Topics

• Alkenes (pharmacology)
• Antineoplastic Agents (pharmacology)
• Biomarkers, Tumor (genetics, metabolism)
• Blotting, Western
• Bone Neoplasms (genetics, metabolism)
• Cell Line, Tumor
• Electrophoresis, Gel, Two-Dimensional
• Humans
• Neoplasm Proteins (genetics, metabolism)
• Osteosarcoma (genetics, metabolism)
• Phenols (pharmacology)
• Proteome (genetics, metabolism)
• Proteomics
• Reverse Transcriptase Polymerase Chain Reaction
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization