IL-18 enhances the migration ability of murine melanoma cells through the generation of ROI and the MAPK pathway.

Abstract

Interleukin-18 (IL-18) has multiple effects on various cells that are involved in immune escape of murine melanoma cells and in the inflammatory responses. This study investigated the effect of IL-18 on the ability of murine melanoma cells to migrate by using B16F10 cells and the IL-18 antisense transfectants of B16F10 cells (the B16F10/IL-18 antisense transfectant). The B16F10 cells were more able to migrate than were the B16F10/IL-18 antisense transfectants. An exogenous IL-18 treatment improved the ability of the B16F10/IL-18 antisense transfectant cells to migrate, indicating that IL-18 enhanced the migration ability of melanoma cells. To determine the signaling mechanisms involved in IL-18-enhanced migration, we measured the ROI levels. It was found that the ROI levels were increased by IL-18, and an antioxidant, N-acetyl-l-cystein (NAC), blocked the effect of IL-18 on migration, suggesting the involvement of ROI in the signal transduction of IL-18-enhanced cell migration. IL-18-enhanced cell migration was also reduced by PD98059. In addition, the level of ERK1/2 phosphorylation was markedly increased by treating with exogenous IL-18 at 20 min. These results suggest that IL-18 enhances the ability of melanoma cells to migrate via the generation of ROI and the MAPK pathway.

• Authors: Min Kyung Jung, Hyun Keun Song, Kyung-Eun Kim, Dae Young Hur, Taesung Kim, Saic Bang, Hyunjeong Park, Dae Ho Cho
• Journal: Immunology letters (Immunol Lett) Vol. 107 Issue 2 Pg. 125-30 (Nov 15 2006) ISSN: 0165-2478 [Print] Netherlands
• PMID: 17014914 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Flavonoids
• Free Radical Scavengers
• Interleukin-18
• Protein Kinase Inhibitors
• Reactive Oxygen Species
• Mitogen-Activated Protein Kinase 1
• Mitogen-Activated Protein Kinase 3
• Mitogen-Activated Protein Kinases
• 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
• Acetylcysteine

Topics

• Acetylcysteine (pharmacology)
• Animals
• Cell Movement (drug effects)
• Flavonoids (pharmacology)
• Free Radical Scavengers (pharmacology)
• Interleukin-18 (genetics, pharmacology, physiology)
• Melanoma, Experimental (immunology, pathology)
• Mice
• Mitogen-Activated Protein Kinase 1 (antagonists & inhibitors, metabolism)
• Mitogen-Activated Protein Kinase 3 (antagonists & inhibitors, metabolism)
• Mitogen-Activated Protein Kinases (antagonists & inhibitors, metabolism)
• Phosphorylation
• Protein Kinase Inhibitors (pharmacology)
• Reactive Oxygen Species (analysis, antagonists & inhibitors, metabolism)
• Transfection