Carboxymethyl chitin-glucan enriched diet exhibits protective effects against oxidative DNA damage induced in freshly isolated rat cells.

Abstract

The connection between dietary intake of carboxymethyl chitin-glucan (CM-CG, approximately 200 mg/kg body weight, during 21 days) and the response of freshly isolated rat cells to genotoxic treatment with a combination of photosensitizer Methylene Blue and visible light (MB+VL) was evaluated in presented study. Blood lymphocytes, testicular cells, and hepatocytes were isolated from rats fed by a standard or CM-CG enriched diet and in ex vivo conditions challenged with oxidative agent. Induced DNA damage was assessed using a modified comet assay. When added to the diet, CM-CG itself did not induce any negative effect on the health condition of animals or on level of DNA breaks in rat cells. Moreover, the cells isolated from CM-CG fed animals were more resistant to oxidative stress induced by visible light-excited Methylene Blue. In conclusion, we have demonstrated that carboxymethyl chitin-glucan represents a natural fungal polysaccharide that is able to exert antimutagenic properties upon application in diet.

• Authors: M Lazarová, J Lábaj, G Kogan, D Slamenová
• Journal: Neoplasma (Neoplasma) Vol. 53 Issue 5 Pg. 434-9 ( 2006) ISSN: 0028-2685 [Print] Slovakia
• PMID: 17013539 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antimutagenic Agents
• Glucans
• Oxidants
• carboxymethyl-chitin-glucan
• Chitin
• Methylene Blue

Topics

• Animals
• Antimutagenic Agents (administration & dosage)
• Cells, Cultured
• Chitin (administration & dosage, analogs & derivatives)
• Comet Assay
• DNA Damage (drug effects)
• Diet
• Glucans (administration & dosage)
• Male
• Methylene Blue (toxicity)
• Oxidants (toxicity)
• Oxidative Stress (drug effects)
• Rats
• Rats, Sprague-Dawley