EDTA-induced urothelial cell shedding for the treatment of superficial bladder cancer in the mouse.

Abstract

AIM:

The aim of this study was to determine the effect of intravesical EDTA instillation on the development of intravesically implanted tumor cells in normal mice.

METHODS:

The mouse bladder tumor (MBT-2) model was used in female C3H/eb mice to evaluate the amount of normal urothelial cell shedding, and the degree of tumor growth inhibition following intravesical EDTA instillation in comparison with phosphate-buffered saline (PBS) instillation.

RESULTS:

At 1 h after instillation, the number of urothelial cells aspirated was 500-1000 per PBS-treated mouse and 10,000-20,000 per EDTA-treated mouse (P < 0.00001). The bladder weight, which reflected the effect of the agent on the tumor, was similar in the untreated and PBS-treated mice (105.46 +/- 46 mg and 106.2 +/- 50 mg, respectively). It was significantly lower in the EDTA-treated mice (80.4 +/- 42 mg) (P = 0.0045).

CONCLUSIONS:

Intravesical administration of EDTA results in significant normal and neoplastic urothelial cell shedding. Intravesical irrigation with EDTA may prevent adherence of the malignant cells to the bladder wall following tumor resection.

• Authors: Ofer Nativ, Eilata Dalal, Guy Hidas, Moshe Aronson
• Journal: International journal of urology : official journal of the Japanese Urological Association (Int J Urol) Vol. 13 Issue 10 Pg. 1344-6 (Oct 2006) ISSN: 0919-8172 [Print] Australia
• PMID: 17010016 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Chelating Agents
• Edetic Acid

Topics

• Administration, Intravesical
• Animals
• Chelating Agents (administration & dosage, therapeutic use)
• Disease Models, Animal
• Disease Progression
• Edetic Acid (administration & dosage, therapeutic use)
• Female
• Mice
• Mice, Inbred C3H
• Neoplasm Transplantation
• Treatment Outcome
• Urinary Bladder (drug effects, pathology)
• Urinary Bladder Neoplasms (etiology, pathology, therapy)
• Urothelium (drug effects, pathology)