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Induction of apoptosis on human hepatocarcinoma cell lines by an alkyl resorcinol isolated from Lithraea molleoides.

AbstractAIM:
To study the mechanism of cytotoxicity of a new active 5-alkyl resorcinol [1, 3-dihydroxy-5- (tridec-4', 7'-dienyl) benzene] isolated from Lithraea molleoides leaves on liver tumor cells.
METHODS:
Human hepatocarcinoma cell lines (HepG2 and Hep3B) in culture were treated with inhibitory concentrations, 50% of the compound, for 24 h. The induction of apoptosis was detected in treated cells by analysis of DNA fragmentation, DNA content, and acridine orange and propidium iodide staining.
RESULTS:
After 24 h of 5-alkyl resorcinol treatment, both cell lines showed: (1) the typical morphological alterations of apoptosis; (2) DNA fragmentation, detected by laddering and appearance of a subG0 population by flow cytometry; and (3) condensed and fragmented nuclei by acridine orange-propidium iodide staining.
CONCLUSION:
Based on the results, this compound exerts its cytotoxic effect in both hepatocellular cell lines through apoptotic cell death. For Hep3B, cells with mutated p53 and Fas, apoptosis would proceed by p53- or Fas-independent pathways.
AuthorsLuciana Barbini, Paula Lopez, Julieta Ruffa, Virginia Martino, Graciela Ferraro, Rodolfo Campos, Lucia Cavallaro
JournalWorld journal of gastroenterology (World J Gastroenterol) Vol. 12 Issue 37 Pg. 5959-63 (Oct 07 2006) ISSN: 1007-9327 [Print] United States
PMID17009393 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • 1,3-dihydroxy-5-(tridec-4',7'-dienyl)benzene
  • Cytotoxins
  • DNA, Neoplasm
  • Fas-Associated Death Domain Protein
  • Plant Extracts
  • Resorcinols
  • Tumor Suppressor Protein p53
Topics
  • Anacardiaceae (chemistry)
  • Apoptosis (drug effects)
  • Carcinoma, Hepatocellular (drug therapy, pathology)
  • Cell Line, Tumor
  • Cell Nucleus (drug effects, pathology)
  • Cytotoxins (analysis, pharmacology, therapeutic use)
  • DNA Fragmentation (drug effects)
  • DNA, Neoplasm (drug effects)
  • Fas-Associated Death Domain Protein (genetics)
  • Humans
  • Liver Neoplasms (drug therapy, pathology)
  • Mutation
  • Plant Extracts (analysis, therapeutic use)
  • Plant Leaves (chemistry)
  • Resorcinols (pharmacology)
  • Resting Phase, Cell Cycle (drug effects)
  • Tumor Suppressor Protein p53 (genetics)

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