[Effect of erythromycin on apoptosis of epithelial cell in nasal polyps].

Abstract

OBJECTIVE:

To study the effect of erythromycin on apoptosis and expression of Bax and Bcl-2 of epithelial cell in nasal polyps.

METHODS:

Epithelial cells from thirty nasal polyps and fifteen inferior turbinates were cultured in Dulbecco Eagle and Ham F12 (1:1) and divided into two groups (one group were treated with Erythromycin). Apoptosis was detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling and expression of Bcl-2 and Bax detected by immunohistochemistry at 1, 3, 5 days after culture.

RESULTS:

The apoptosis indexes (AI) of epithelial cell in nasal polyps after cultured for 1, 3, 5 days with erythromycin were (33.23 +/- 6.50)%, (38.21 +/- 7.22)% and (52.63 +/- 7.86)% respectively. The AI of epithelial cell in inferior turbinates were (31.02 +/- 5.60)%, (32.13 +/- 7.15)% and (39.64 +/- 7.48)% respectively. There was significant difference between two groups at 5 day after culture (P < 0.05). Expression of Bax and Bcl-2 of epithelial cell was significantly higher in nasal polyps than that in inferior turbinates (P < 0.01). Expression of Bax of epithelial cell cultured with erythromycin was significantly higher than that in the controls (P < 0.05). Apoptosis was clearly promoted after 5 day culture with Erythromycin (P < 0.05).

CONCLUSIONS:

Erythromycin promoted expression of Bax and apoptosis of epithelial cell in nasal polyps.

• Authors: Sai-Nan Xu, Yu Feng, Yu Zhang, Yi-Fei Zhou, Lei Yang, Yong-Guo Jin
• Journal: Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery (Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi) Vol. 41 Issue 7 Pg. 510-3 (Jul 2006) ISSN: 1673-0860 [Print] China
• PMID: 17007374 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Proto-Oncogene Proteins c-bcl-2
• bcl-2-Associated X Protein
• Erythromycin

Topics

• Adolescent
• Adult
• Apoptosis (drug effects)
• Case-Control Studies
• Cell Line
• Epithelial Cells (drug effects, metabolism)
• Erythromycin (pharmacology)
• Female
• Humans
• Male
• Middle Aged
• Nasal Polyps (metabolism, pathology)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Young Adult
• bcl-2-Associated X Protein (metabolism)