Cytoprotective effects of the antioxidant phytochemical indicaxanthin in beta-thalassemia red blood cells.

Abstract

Antioxidant phytochemicals are investigated as novel treatments for supportive therapy in beta-thalassemia. The dietary indicaxanthin was assessed for its protective effects on human beta-thalassemic RBCs submitted in vitro to oxidative haemolysis by cumene hydroperoxide. Indicaxanthin at 1.0-10 microM enhanced the resistance to haemolysis dose-dependently. In addition, it prevented lipid and haemoglobin (Hb) oxidation, and retarded vitamin E and GSH depletion. After ex vivo spiking of blood from thalassemia patients with indicaxanthin, the phytochemical was recovered in the soluble cell compartment of the RBCs. A spectrophotometric study showed that indicaxanthin can reduce perferryl-Hb generated in solution from met-Hb and hydrogen peroxide (H2O2), more effectively than either Trolox or vitamin C. Collectively our results demonstrate that indicaxanthin can be incorporated into the redox machinery of beta-thalassemic RBC and defend the cell from oxidation, possibly interfering with perferryl-Hb, a reactive intermediate in the hydroperoxide-dependent Hb degradation. Opportunities of therapeutic interest for beta-thalassemia may be considered.

• Authors: L Tesoriere, M Allegra, D Butera, C Gentile, M A Livrea
• Journal: Free radical research (Free Radic Res) Vol. 40 Issue 7 Pg. 753-61 (Jul 2006) ISSN: 1071-5762 [Print] England
• PMID: 16984002 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Benzene Derivatives
• Betaxanthins
• Hemoglobins
• Pyridines
• indicaxanthin
• Vitamin E
• Hemin
• Glutathione
• cumene hydroperoxide

Topics

• Antioxidants (metabolism, pharmacology)
• Benzene Derivatives (toxicity)
• Betaxanthins (metabolism, pharmacology)
• Case-Control Studies
• Cytoprotection
• Dose-Response Relationship, Drug
• Erythrocytes (drug effects, metabolism)
• Glutathione (blood)
• Hemin (metabolism)
• Hemoglobins (metabolism)
• Hemolysis
• Humans
• Lipid Metabolism
• Oxidation-Reduction
• Pyridines (metabolism, pharmacology)
• Spectrophotometry
• Vitamin E (blood)
• beta-Thalassemia (blood)