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Hindlimb unloading decreases thioredoxin-related antioxidant proteins and increases thioredoxin-binding protein-2 in rat skeletal muscle.

Abstract
To investigate role(s) of thioredoxin-related antioxidant proteins in disuse muscle atrophy, we examined the levels of thioredoxin-1 (Trx-1), peroxiredoxin-3/SP-22 (Prx-3) and thioredoxin-binding protein-2 (TBP-2) in rat soleus muscle subjected to hindlimb unloading (HU) for 2, 4, 7 or 14 days. The muscle weight loss was initially observed on day 4. The increases in aclorein- and malondialdehyde-modified proteins, and the decreases in the levels of Trx-1, Prx-3 and Mn-SOD were observed in the late phase of muscle atrophy, whereas, the increase in mRNA expression of TBP-2, a negative regulator of thioredoxin, preceded muscle atrophy. These findings suggest that the decrease of those antioxidant proteins, particularly a marked decrease of Trx-1, may be responsible for the enhanced oxidative damage during the late phase of disuse muscle atrophy. Furthermore, the increase in TBP-2 preceding the muscle atrophy may suppress the thioredoxin-mediated redox signaling, which can be an initial trigger leading to disuse muscle atrophy.
AuthorsYasuyuki Matsushima, Hiroki Nanri, Soichiro Nara, Tatsuya Okufuji, Masanori Ohta, Kenji Hachisuka, Masaharu Ikeda
JournalFree radical research (Free Radic Res) Vol. 40 Issue 7 Pg. 715-22 (Jul 2006) ISSN: 1071-5762 [Print] England
PMID16983998 (Publication Type: Journal Article)
Chemical References
  • Carrier Proteins
  • RNA, Messenger
  • Txn1 protein, rat
  • thioredoxin-binding protein-2, rat
  • Malondialdehyde
  • Thioredoxins
  • Peroxidases
  • Peroxiredoxins
  • Superoxide Dismutase
Topics
  • Animals
  • Blotting, Western
  • Carrier Proteins (genetics, metabolism)
  • Hindlimb Suspension
  • Male
  • Malondialdehyde
  • Muscle, Skeletal (metabolism)
  • Muscular Atrophy (metabolism)
  • Oxidative Stress
  • Peroxidases (genetics, metabolism)
  • Peroxiredoxins
  • Protein Processing, Post-Translational
  • RNA, Messenger (genetics, metabolism)
  • Rats
  • Rats, Wistar
  • Reverse Transcriptase Polymerase Chain Reaction
  • Superoxide Dismutase (metabolism)
  • Thioredoxins (genetics, metabolism)

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