Abstract |
To investigate role(s) of thioredoxin-related antioxidant proteins in disuse muscle atrophy, we examined the levels of thioredoxin-1 (Trx-1), peroxiredoxin-3/SP-22 (Prx-3) and thioredoxin-binding protein-2 (TBP-2) in rat soleus muscle subjected to hindlimb unloading (HU) for 2, 4, 7 or 14 days. The muscle weight loss was initially observed on day 4. The increases in aclorein- and malondialdehyde-modified proteins, and the decreases in the levels of Trx-1, Prx-3 and Mn-SOD were observed in the late phase of muscle atrophy, whereas, the increase in mRNA expression of TBP-2, a negative regulator of thioredoxin, preceded muscle atrophy. These findings suggest that the decrease of those antioxidant proteins, particularly a marked decrease of Trx-1, may be responsible for the enhanced oxidative damage during the late phase of disuse muscle atrophy. Furthermore, the increase in TBP-2 preceding the muscle atrophy may suppress the thioredoxin-mediated redox signaling, which can be an initial trigger leading to disuse muscle atrophy.
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Authors | Yasuyuki Matsushima, Hiroki Nanri, Soichiro Nara, Tatsuya Okufuji, Masanori Ohta, Kenji Hachisuka, Masaharu Ikeda |
Journal | Free radical research
(Free Radic Res)
Vol. 40
Issue 7
Pg. 715-22
(Jul 2006)
ISSN: 1071-5762 [Print] England |
PMID | 16983998
(Publication Type: Journal Article)
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Chemical References |
- Carrier Proteins
- RNA, Messenger
- Txn1 protein, rat
- thioredoxin-binding protein-2, rat
- Malondialdehyde
- Thioredoxins
- Peroxidases
- Peroxiredoxins
- Superoxide Dismutase
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Topics |
- Animals
- Blotting, Western
- Carrier Proteins
(genetics, metabolism)
- Hindlimb Suspension
- Male
- Malondialdehyde
- Muscle, Skeletal
(metabolism)
- Muscular Atrophy
(metabolism)
- Oxidative Stress
- Peroxidases
(genetics, metabolism)
- Peroxiredoxins
- Protein Processing, Post-Translational
- RNA, Messenger
(genetics, metabolism)
- Rats
- Rats, Wistar
- Reverse Transcriptase Polymerase Chain Reaction
- Superoxide Dismutase
(metabolism)
- Thioredoxins
(genetics, metabolism)
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