Abstract | OBJECTIVE: DESIGN:
Allergic rhinitis symptom data were prospectively collected in the form of the Rhinitis Symptom Utility Index, the Rhinoconjunctivitis Quality of Life Questionnaire, and a Visual Analog Scale. Immunohistochemical staining for SP-A was performed on resected nasal tissue. Quantitative polymerase chain reaction amplification of the SP-A gene referenced to beta-actin was performed on complementary DNA samples synthesized from total RNA isolates. SETTING: Academic tertiary referral center, department of otolaryngology laboratories. PATIENTS: MAIN OUTCOME MEASURES: Immunohistochemical staining of SP-A in human nasal mucosa and submucosa, polymerase chain reaction amplification of SP-A messenger RNA, and rhinitis symptom scores. RESULTS: Immunostaining localized SP-A to the mucosa and submucosal glands in specimens. Quantitative polymerase chain reaction demonstrated correlation between SP-A messenger RNA concentration and the total Rhinitis Symptom Utility Index score (0.51, P = .009) as well as " sneezing over the previous week" (0.40, P = .049), " runny nose over the previous week" (0.55, P = .005), and " sneezing today" (0.47, P = .02). CONCLUSIONS: To our knowledge, this is the first report of SP-A expression in human nasal tissue. Furthermore, the degree of expression correlated with severity of disease as measured by the Rhinitis Symptom Utility Index in patients with allergic rhinitis symptoms.
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Authors | Christopher T Wootten, Robert F Labadie, Anton Chen, Kirk F Lane |
Journal | Archives of otolaryngology--head & neck surgery
(Arch Otolaryngol Head Neck Surg)
Vol. 132
Issue 9
Pg. 1001-7
(Sep 2006)
ISSN: 0886-4470 [Print] United States |
PMID | 16982978
(Publication Type: Journal Article)
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Chemical References |
- Pulmonary Surfactant-Associated Protein A
- Pulmonary Surfactants
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Topics |
- Adult
- Female
- Humans
- Immunohistochemistry
- Male
- Nasal Mucosa
(metabolism)
- Polymerase Chain Reaction
- Pulmonary Surfactant-Associated Protein A
(metabolism)
- Pulmonary Surfactants
(metabolism)
- Rhinitis, Allergic, Perennial
(metabolism)
- Rhinitis, Allergic, Seasonal
(metabolism)
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