[Expression of eosinophil major basic protein mRNA in bronchial asthma].

Abstract

OBJECTIVE:

To investigate the expression of eosinophil major basic protein (MBP) mRNA in bronchial asthma and explore its significance.

METHODS:

Peripheral blood eosinophil MBP mRNA levels were measured in 40 patients with asthma and 20 normal controls by semi-quantitative RT-PCR. The association of MBP mRNA levels with eosinophil count and pulmonary function was also analyzed.

RESULTS:

Compared with the normal control group, MBP mRNA level were significantly increased in asthma patients (0.37-/+0.11 vs 0.17-/+0.04, P<0.001), so was the eosinophil count (0.86-/+0.52 vs 0.21-/+0.10, P<0.001). MBP mRNA levels in patients with moderate persistent asthma (0.42-/+0.05) and those with severe persistent asthma (0.47-/+0.05) were significantly higher than those in patients with mild persistent asthma (0.25-/+0.06, P<0.001), and the difference in MBP mRNA levels between moderate persistent asthma patients and severe ones was also significant (P<0.05). Among the asthma patients, MBP mRNA levels showed an inverse correlation with pulmonary function (r=-0.7490, P<0.001).

CONCLUSION:

Increased MBP mRNA expression level may correlate with the severity of asthma. MBP may play an important role in the development of asthma.

• Authors: Shuang-hong Li, Ya-ling Luo, Wen-yan Lai
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 26 Issue 9 Pg. 1330-3 (Sep 2006) ISSN: 1673-4254 [Print] China
• PMID: 16982448 (Publication Type: English Abstract, Journal Article)

Chemical References

• Blood Proteins
• Proteoglycans
• RNA, Messenger
• PRG2 protein, human
• Eosinophil Major Basic Protein

Topics

• Adult
• Asthma (genetics, pathology)
• Blood Proteins (genetics)
• Eosinophil Major Basic Protein
• Female
• Humans
• Male
• Middle Aged
• Proteoglycans (genetics)
• RNA, Messenger (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction