| Abstract | The Sertoli cells play an essential role in the maintenance and control of spermatogenesis. The ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase) and 5'-nucleotidase activities can modulate the extracellular adenine nucleotide levels, controlling nucleotide-mediated signaling events in Sertoli cells. Since thyroid hormones (TH) and adenine nucleotides and nucleosides play important modulatory roles in Sertoli cell proliferation and differentiation, the aim of our study was to investigate the effect of hypothyroidism upon the NTPDase and 5'-nucleotidase activities in Sertoli cell cultures, as well as to verify whether these effects may be reversed by short and long-term supplementation with TH. Congenital hypothyroidism was induced by adding 0.02% methimazole in the drinking water from day 9 of gestation and continually until 18 days of age. Hypothyroidism significantly decreased the extracellular ATP and ADP hydrolysis and this effect was significantly reversed when cell cultures were supplemented with 1 microM T3 or 0.1 microM T4 for 30 min. In contrast, AMP hydrolysis was not altered by hypothyroidism, but was increased by T4 supplementation for 24 h. The presence of the enzymes NTPDase 1, 2 and 3 was detected by RT-PCR in Sertoli cell cultures, however, hypothyroidism was not able to alter the expression of these enzymes. These findings demonstrate that TH modify NTPDase activities in hypothyroid Sertoli cells, probably via nongenomic mechanisms and, consequently, may influence the reproductive function throughout development. |
| Authors | Ariane Zamoner, Alessandra Nejar Bruno, Emerson André Casali, Patrícia Frasson Corbelini, Gabriela Placoná Diniz, Maria Luiza Morais Barreto-Chaves, Fátima Regina Mena Barreto Silva, João José Freitas Sarkis, Regina Pessoa-Pureur
(Affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Porto Alegre RS, Brazil.)
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| Journal | Life sciences
(Life Sci)
Vol. 80
Issue 1
Pg. 51-8
(Dec 3 2006)
ISSN: 0024-3205 England |
| PMID | 16978660
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Antigens, CD
- Thyroid Hormones
- Adenosine Triphosphate
- Adenosine Diphosphate
- Adenosine Triphosphatases
- Pyrophosphatases
- ectoATPase
- nucleoside-triphosphate diphosphohydrolase 3
- Apyrase
- CD39 antigen
|
| Topics |
- Adenosine Diphosphate
(metabolism)
- Adenosine Triphosphatases
(genetics, metabolism)
- Adenosine Triphosphate
(metabolism)
- Animals
- Antigens, CD
(genetics, metabolism)
- Apyrase
(genetics, metabolism)
- Cells, Cultured
- Hypothyroidism
(enzymology)
- Male
- Pyrophosphatases
(genetics, metabolism)
- Rats
- Rats, Wistar
- Sertoli Cells
(enzymology)
- Thyroid Hormones
(physiology)
|
