Synthesis, interaction with double-helical DNA and biological activity of the water soluble complex cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP).

Abstract

The effects exerted by the new complex cis-dichloro-1,2-propylenediaminetetraacetato ruthenium (III), H[RuCl(2)(PDTA-H(2))] [1, RAP], on DNA and cultured tumor cells (ovarian carcinoma TG cell line) were studied. The comparative study of circular dichroism (CD) spectra obtained from DNA and RAP-DNA system evidences the interaction of the complex with DNA. Compound 1 also interacted with tumor TG cells to slow their proliferation rate. BrdU incorporation was enhanced in cells treated with compound 1, as evidenced by a single-cell electrophoresis method (comet assay), in accordance with RAP-induced DNA damage. DNA migration of compound 1-treated cells was similar to that induced by noxious agents other than cross-linking chemicals. The stability of [RuCl(2)(PDTA-H(2))]-DNA binding is suggested by the high degree of damage that persisted after removal of compound 1 from the culture medium.

• Authors: Rosario A Vilaplana, Fátima Delmani, Consolación Manteca, José Torreblanca, Javier Moreno, Gregorio García-Herdugo, Francisco González-Vílchez
• Journal: Journal of inorganic biochemistry (J Inorg Biochem) Vol. 100 Issue 11 Pg. 1834-41 (Nov 2006) ISSN: 0162-0134 [Print] United States
• PMID: 16959320 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Organometallic Compounds
• Ruthenium
• DNA

Topics

• Antineoplastic Agents (chemistry, pharmacology)
• Cell Proliferation (drug effects)
• Circular Dichroism
• Comet Assay (methods)
• DNA (chemistry, genetics)
• DNA Damage
• Humans
• Organometallic Compounds (chemistry, pharmacology)
• Plasmids (chemistry, genetics)
• Ruthenium (chemistry)
• Spectrophotometry, Infrared
• Tumor Cells, Cultured