Abstract | PURPOSE: EXPERIMENTAL DESIGN: The expression of FABP6 mRNA was evaluated in 78 paired samples of cancer/normal tissue representing colorectal cancer cases, plus 16 adenomas, and 16 metastatic lymph nodes. An immunohistochemical study was conducted with paraffin sections. In vitro transfection was done to determine FABP6's biological roles. RESULTS: The expression of FABP6 mRNA was significantly higher in cancer (75 of 78, 96.2%) than in normal tissue (P<0.001). The expression of mRNA was increased in cancer compared with adenoma, but was dramatically decreased in node metastases. Tumors with high FABP6 expression were smaller in size (P<0.01), more often in the left colon (P<0.05), and had shallower invasion into the bowel wall (P<0.05) compared with those with low expression. There was no significant difference between high- and low-expression tumors regarding clinicopathologic variables such as histologic type, lymph node, or liver metastasis, Dukes' classification, and prognosis. Immunohistochemical study revealed that FABP6 expression was primarily observed in cancer cells. In vitro transfection revealed that transfectants showed weaker invasiveness (P<0.05), more dominant proliferation (P<0.001), and less apoptosis than mock cells. CONCLUSIONS:
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Authors | Takahiro Ohmachi, Hiroshi Inoue, Koshi Mimori, Fumiaki Tanaka, Atsushi Sasaki, Tatsuo Kanda, Hiroshi Fujii, Katsuhiko Yanaga, Masaki Mori |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 12
Issue 17
Pg. 5090-5
(Sep 01 2006)
ISSN: 1078-0432 [Print] United States |
PMID | 16951225
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Fatty Acid-Binding Proteins
- Gastrointestinal Hormones
- RNA, Messenger
- fatty acid-binding protein 6
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Topics |
- Adenoma
(genetics, pathology)
- Aged
- Colorectal Neoplasms
(genetics, pathology)
- Fatty Acid-Binding Proteins
(biosynthesis, genetics)
- Female
- Gastrointestinal Hormones
- Gene Expression Profiling
- Humans
- Immunohistochemistry
- Lymph Nodes
(pathology)
- Male
- RNA, Messenger
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
(methods)
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