Abstract |
Previously, we demonstrated the inhibitory effects of Si-Ni-San, a traditional Chinese prescription, on picryl chloride-induced ear contact sensitivity (PCl-CS). This study aimed to evaluate the role of the four major constituents contained in the prescription ( saikosaponins, paeoniflorin, naringin and glycyrrhizin) in the inhibitory effect. When administered during the induction phase, saikosaponin a and glycyrrhizin showed significant inhibitory effects, while paeoniflorin and naringin did not. These components in Si-Ni-San also inhibited the activation and proliferation of T lymphocytes as well as the production of cytokines such as tumour necrosis factor-alpha and interferon-gamma to different extents. Saikosaponin a and paeoniflorin dose-dependently reduced the splenocyte adhesion to type I collagen, while glycyrrhizin only showed a slight tendency. Furthermore, treatment with glycyrrhizin or saikosaponin a, rather than paeoniflorin or naringin, moderately inhibited the matrix metalloproteinase (MMP)-2 activity of the splenocytes from PCl-CS mice, and the combination of all four components showed a strong inhibition against MMP-2. Moreover, the components markedly decreased the serum level of nitric oxide in PCl-sensitized mice. The results indicated that saikosaponin a and glycyrrhizin may be the major contributors in the alleviation effect of Si-Ni-San on contact sensitivity, and paeoniflorin and naringin may exhibit a co-operative effect.
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Authors | Li Zhang, Yi Dong, Yang Sun, Ting Chen, Qiang Xu |
Journal | The Journal of pharmacy and pharmacology
(J Pharm Pharmacol)
Vol. 58
Issue 9
Pg. 1257-64
(Sep 2006)
ISSN: 0022-3573 [Print] England |
PMID | 16945185
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Benzoates
- Bridged-Ring Compounds
- Drugs, Chinese Herbal
- Flavanones
- Glucosides
- Immunosuppressive Agents
- Monoterpenes
- RNA, Messenger
- Saponins
- shigyaku-san
- Concanavalin A
- peoniflorin
- Nitric Oxide
- Glycyrrhizic Acid
- Oleanolic Acid
- Interferon-gamma
- Matrix Metalloproteinase 2
- naringin
- saikosaponin D
- Picryl Chloride
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Topics |
- Animals
- Benzoates
(pharmacology)
- Bridged-Ring Compounds
(pharmacology)
- Cell Adhesion
(drug effects)
- Cells, Cultured
- Concanavalin A
- Dermatitis, Allergic Contact
(blood, enzymology, immunology, prevention & control)
- Dose-Response Relationship, Drug
- Drugs, Chinese Herbal
(chemistry, pharmacology)
- Female
- Flavanones
(pharmacology)
- Glucosides
(pharmacology)
- Glycyrrhizic Acid
(pharmacology)
- Immunosuppressive Agents
(chemistry, pharmacology)
- Interferon-gamma
(biosynthesis, genetics)
- Lymphocyte Activation
(drug effects)
- Lymphocytes
(drug effects, enzymology, immunology)
- Matrix Metalloproteinase 2
(metabolism)
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Mice, Inbred ICR
- Monoterpenes
- Nitric Oxide
(blood)
- Oleanolic Acid
(analogs & derivatives, pharmacology)
- Picryl Chloride
- RNA, Messenger
(biosynthesis)
- Saponins
(pharmacology)
- Spleen
(cytology, drug effects, enzymology, immunology)
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